ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers

Yuqin Zhang, Himabindu Vasuki Kilambi, Jie Liu, Hamutal Bar, Shani Lazary, Aiman Egbaria, Dagmar Ripper, Laurence Charrier, Zeinu Mussa Belew, Nikolai Wulff, Suresh Damodaran, Hussam Hassan Nour-Eldin, Asaph Aharoni, Laura Ragni, Lucia Strader, Nir Sade, Roy Weinstain, Markus Geisler, Eilon Shani

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of abscisic acid (ABA) on plant growth, development, and response to the environment depend on local ABA concentrations. Here, we show that in Arabidopsis, ABA homeostasis is regulated by two previously unknown ABA transporters. Adenosine triphosphate-binding cassette subfamily G member 17 (ABCG17) and ABCG18 are localized to the plasma membranes of leaf mesophyll and cortex cells to redundantly promote ABA import, leading to conjugated inactive ABA sinks, thus restricting stomatal closure. ABCG17 and ABCG18 double knockdown revealed that the transporters encoded by these genes not only limit stomatal aperture size, conductance, and transpiration while increasing water use efficiency but also control ABA translocation from the shoot to the root to regulate lateral root emergence. Under abiotic stress conditions, ABCG17 and ABCG18 are transcriptionally repressed, promoting active ABA movement and response. The transport mechanism mediated by ABCG17 and ABCG18 allows plants to maintain ABA homeostasis under normal growth conditions.

Original languageEnglish
Article numbereabf6069
JournalScience advances
Volume7
Issue number43
DOIs
StatePublished - Oct 2021

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