TY - JOUR
T1 - A VIP antagonist distinguishes VIP receptors on spinal cord cells and lymphocytes
AU - Gozes, Yehoshua
AU - Brenneman, Douglas E.
AU - Fridkin, Mati
AU - Asofsky, Richard
AU - Gozes, Illana
PY - 1991/2/1
Y1 - 1991/2/1
N2 - Vasoactive intestinal peptide (VIP) is a neuropeptide which also interacts with cells of the immune system. The paucity of specific VIP receptor antagonists has hampered studies of possible receptor heterogeneity and of VIP function. To aid in achieving these goals, a new VIP antagonist, a hybrid between neurotensin and VIP, has been synthesized. This peptide interacted with VIP receptors on spinal cord cells with an affinity 10-fold greater than VIP itself. In contrast, 1000-fold higher concentrations of the antagonist were required to displace labeled VIP from its receptor on lymphoid cells as compared to VIP itself, suggesting VIP receptor heterogeneity between immune and spinal cord cells.
AB - Vasoactive intestinal peptide (VIP) is a neuropeptide which also interacts with cells of the immune system. The paucity of specific VIP receptor antagonists has hampered studies of possible receptor heterogeneity and of VIP function. To aid in achieving these goals, a new VIP antagonist, a hybrid between neurotensin and VIP, has been synthesized. This peptide interacted with VIP receptors on spinal cord cells with an affinity 10-fold greater than VIP itself. In contrast, 1000-fold higher concentrations of the antagonist were required to displace labeled VIP from its receptor on lymphoid cells as compared to VIP itself, suggesting VIP receptor heterogeneity between immune and spinal cord cells.
KW - Lymphocyte
KW - Spinal cord cell culture
KW - Vasoactive intestinal peptide
KW - Vasoactive intestinal peptide receptor antagonist
KW - Vasoactive intestinal receptor
UR - http://www.scopus.com/inward/record.url?scp=0026100931&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(91)90528-4
DO - 10.1016/0006-8993(91)90528-4
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AN - SCOPUS:0026100931
SN - 0006-8993
VL - 540
SP - 319
EP - 321
JO - Brain Research
JF - Brain Research
IS - 1-2
ER -