TY - JOUR
T1 - A Vibrio T6SS-Mediated Lethality in an Aquatic Animal Model
AU - Cohen, Hadar
AU - Fridman, Chaya M.
AU - Gerlic, Motti
AU - Salomon, Dor
N1 - Publisher Copyright:
© 2023 Cohen et al.
PY - 2023/8
Y1 - 2023/8
N2 - Bacteria belonging to the genus Vibrio include many known and emerging pathogens. Horizontal gene transfer of pathogenicity islands is a major contributor to the emergence of new pathogenic Vibrio strains. Here, we use the brine shrimp Artemia salina as a model and show that the marine bacterium Vibrio proteolyticus uses a horizontally shared type VI secretion system, T6SS3, to intoxicate a eukaryotic host. Two T6SS3 effectors, which were previously shown to induce inflammasome-mediated pyroptotic cell death in mammalian phagocytic cells, contribute to this toxicity. Furthermore, we find a novel T6SS3 effector that also contributes to the lethality mediated by this system against Artemia salina. Therefore, our results reveal a T6SS that is shared among diverse vibrios and mediates host lethality, indicating that it can lead to the emergence of new pathogenic strains.
AB - Bacteria belonging to the genus Vibrio include many known and emerging pathogens. Horizontal gene transfer of pathogenicity islands is a major contributor to the emergence of new pathogenic Vibrio strains. Here, we use the brine shrimp Artemia salina as a model and show that the marine bacterium Vibrio proteolyticus uses a horizontally shared type VI secretion system, T6SS3, to intoxicate a eukaryotic host. Two T6SS3 effectors, which were previously shown to induce inflammasome-mediated pyroptotic cell death in mammalian phagocytic cells, contribute to this toxicity. Furthermore, we find a novel T6SS3 effector that also contributes to the lethality mediated by this system against Artemia salina. Therefore, our results reveal a T6SS that is shared among diverse vibrios and mediates host lethality, indicating that it can lead to the emergence of new pathogenic strains.
KW - Artemia
KW - Vibrio proteolyticus
KW - effector
KW - effector functions
KW - host-pathogen interaction
KW - secretion
KW - virulence
UR - http://www.scopus.com/inward/record.url?scp=85168248391&partnerID=8YFLogxK
U2 - 10.1128/spectrum.01093-23
DO - 10.1128/spectrum.01093-23
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C2 - 37310285
AN - SCOPUS:85168248391
SN - 2165-0497
VL - 11
JO - Microbiology spectrum
JF - Microbiology spectrum
IS - 4
ER -