A U1/U4/U5 snRNP complex induced by a 2′-O-methyl-oligoribonucleotide complementary to U5 snRNA

Gil Ast, Alan M. Weiner

Research output: Contribution to journalArticlepeer-review

Abstract

Nuclear messenger RNA splicing involves multiple interactions between the five spliceosomal small nuclear ribonucleoprotein particles (snRNPs) U1, U2, U4, U5, and U6 and numerous spliceosomal proteins. Here it is shown that binding of a 2′-O-methyl-oligoribonucleotide complementary to U5 small nuclear RNA (snRNA) nucleotides 68 to 88 (BU5Ae) disrupts the initial U4/U5/U6 tri-snRNP complex, enhances the U2/U6 interaction, and induces a U1/U4/U5 snRNP complex. The U1/U4/U5 snRNP complex interacts specifically with an RNA oligonucleotide containing the 5′ splice site sequence and may therefore represent a transitional stage in the displacement of U1 from the 5′ splice site by U5 snRNP.

Original languageEnglish
Pages (from-to)881-884
Number of pages4
JournalScience
Volume272
Issue number5263
DOIs
StatePublished - 1996
Externally publishedYes

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