A two-phase protocol for ambient hydrogen production using Chlamydomonas reinhardtii

Tamar Elman, Iftach Yacoby*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


H2 production from green-microalgae, for energy purposes, is the ultimate goal of large-scale production. Here, we present a two-phase protocol for hydrogen production assay under ambient conditions using Chlamydomonas reinhardtii, which eliminates steps used previously, including centrifugation and resuspension with sulfur-deprived media. We detail steps for Chlamydomonas reinhardtii culture, acetate supply replenishment, anaerobic induction, and H2 quantification. This protocol enables large-scale experiments in an easy and cost-effective method while maintaining cells vital, crucial factors for transition to industrial scales. For complete details on the use and execution of this protocol, please refer to Elman et al. (2022).

Original languageEnglish
Article number101640
JournalSTAR Protocols
Issue number3
StatePublished - 16 Sep 2022


  • Biotechnology and bioengineering
  • Energy
  • Microbiology
  • Model organisms


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