TY - JOUR
T1 - A systematic view on influenza induced host shutoff
AU - Bercovich-Kinori, Adi
AU - Tai, Julie
AU - Gelbart, Idit Anna
AU - Shitrit, Alina
AU - Ben-Moshe, Shani
AU - Drori, Yaron
AU - Itzkovitz, Shalev
AU - Mandelboim, Michal
AU - Stern-Ginossar, Noam
N1 - Publisher Copyright:
© Bercovich-Kinori et al.
PY - 2016/8/15
Y1 - 2016/8/15
N2 - Host shutoff is a common strategy used by viruses to repress cellular mRNA translation and concomitantly allow the efficient translation of viral mRNAs. Here we use RNA-sequencing and ribosome profiling to explore the mechanisms that are being utilized by the Influenza A virus (IAV) to induce host shutoff. We show that viral transcripts are not preferentially translated and instead the decline in cellular protein synthesis is mediated by viral takeover on the mRNA pool. Our measurements also uncover strong variability in the levels of cellular transcripts reduction, revealing that short transcripts are less affected by IAV. Interestingly, these mRNAs that are refractory to IAV infection are enriched in cell maintenance processes such as oxidative phosphorylation. Furthermore, we show that the continuous oxidative phosphorylation activity is important for viral propagation. Our results advance our understanding of IAV-induced shutoff, and suggest a mechanism that facilitates the translation of genes with important housekeeping functions.
AB - Host shutoff is a common strategy used by viruses to repress cellular mRNA translation and concomitantly allow the efficient translation of viral mRNAs. Here we use RNA-sequencing and ribosome profiling to explore the mechanisms that are being utilized by the Influenza A virus (IAV) to induce host shutoff. We show that viral transcripts are not preferentially translated and instead the decline in cellular protein synthesis is mediated by viral takeover on the mRNA pool. Our measurements also uncover strong variability in the levels of cellular transcripts reduction, revealing that short transcripts are less affected by IAV. Interestingly, these mRNAs that are refractory to IAV infection are enriched in cell maintenance processes such as oxidative phosphorylation. Furthermore, we show that the continuous oxidative phosphorylation activity is important for viral propagation. Our results advance our understanding of IAV-induced shutoff, and suggest a mechanism that facilitates the translation of genes with important housekeeping functions.
UR - http://www.scopus.com/inward/record.url?scp=84988535398&partnerID=8YFLogxK
U2 - 10.7554/eLife.18311
DO - 10.7554/eLife.18311
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C2 - 27525483
AN - SCOPUS:84988535398
SN - 2050-084X
VL - 5
JO - eLife
JF - eLife
IS - AUGUST
M1 - e18311
ER -