A specific subset of mouse bone marrow cells has smooth muscle cell differentiation capacity-brief report

Pat Metharom; Arun H.S. Kumar; Sharon Weiss; Noel M. Caplice

doi:10.1161/ATVBAHA.109.200097

A specific subset of mouse bone marrow cells has smooth muscle cell differentiation capacity-brief report

Pat Metharom, Arun H.S. Kumar, Sharon Weiss, Noel M. Caplice

University College Cork

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Objective-To determine whether CX3CR1 bone marrow cells have the capacity for smooth muscle cell (SMC) differentiation. Methods and Results-CX3CR1 and CX3CR1 cells were isolated from marrow of CX3CR1 transgenic mice and cultured in SMC differentiation media. Phenotypic and functional analyses showed only CX3CR1 bone marrow cells exhibit colony cell outgrowth with SMC-specific protein expression, calcium signaling, and contraction responses similar to mature SMC. Conclusion-CX3CR1 marks a bone marrow cells population that enriches for progenitors with capacity to differentiate in vitro into SMC-like cells.

Original language	English
Pages (from-to)	533-535
Number of pages	3
Journal	Arteriosclerosis, Thrombosis, and Vascular Biology
Volume	30
Issue number	3
DOIs	https://doi.org/10.1161/ATVBAHA.109.200097
State	Published - Mar 2010
Externally published	Yes

Keywords

Bone marrow
CX3CR1
Differentiation
Smooth muscle

Access to Document

10.1161/ATVBAHA.109.200097

Cite this

@article{b7becf6806a04373b933ed1d84be0c4e,

title = "A specific subset of mouse bone marrow cells has smooth muscle cell differentiation capacity-brief report",

abstract = "Objective-To determine whether CX3CR1 bone marrow cells have the capacity for smooth muscle cell (SMC) differentiation. Methods and Results-CX3CR1 and CX3CR1 cells were isolated from marrow of CX3CR1 transgenic mice and cultured in SMC differentiation media. Phenotypic and functional analyses showed only CX3CR1 bone marrow cells exhibit colony cell outgrowth with SMC-specific protein expression, calcium signaling, and contraction responses similar to mature SMC. Conclusion-CX3CR1 marks a bone marrow cells population that enriches for progenitors with capacity to differentiate in vitro into SMC-like cells.",

keywords = "Bone marrow, CX3CR1, Differentiation, Smooth muscle",

author = "Pat Metharom and Kumar, {Arun H.S.} and Sharon Weiss and Caplice, {Noel M.}",

year = "2010",

month = mar,

doi = "10.1161/ATVBAHA.109.200097",

language = "אנגלית",

volume = "30",

pages = "533--535",

journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",

issn = "1079-5642",

publisher = "Lippincott Williams and Wilkins Ltd.",

number = "3",

}

TY - JOUR

T1 - A specific subset of mouse bone marrow cells has smooth muscle cell differentiation capacity-brief report

AU - Metharom, Pat

AU - Kumar, Arun H.S.

AU - Weiss, Sharon

AU - Caplice, Noel M.

PY - 2010/3

Y1 - 2010/3

N2 - Objective-To determine whether CX3CR1 bone marrow cells have the capacity for smooth muscle cell (SMC) differentiation. Methods and Results-CX3CR1 and CX3CR1 cells were isolated from marrow of CX3CR1 transgenic mice and cultured in SMC differentiation media. Phenotypic and functional analyses showed only CX3CR1 bone marrow cells exhibit colony cell outgrowth with SMC-specific protein expression, calcium signaling, and contraction responses similar to mature SMC. Conclusion-CX3CR1 marks a bone marrow cells population that enriches for progenitors with capacity to differentiate in vitro into SMC-like cells.

AB - Objective-To determine whether CX3CR1 bone marrow cells have the capacity for smooth muscle cell (SMC) differentiation. Methods and Results-CX3CR1 and CX3CR1 cells were isolated from marrow of CX3CR1 transgenic mice and cultured in SMC differentiation media. Phenotypic and functional analyses showed only CX3CR1 bone marrow cells exhibit colony cell outgrowth with SMC-specific protein expression, calcium signaling, and contraction responses similar to mature SMC. Conclusion-CX3CR1 marks a bone marrow cells population that enriches for progenitors with capacity to differentiate in vitro into SMC-like cells.

KW - Bone marrow

KW - CX3CR1

KW - Differentiation

KW - Smooth muscle

UR - http://www.scopus.com/inward/record.url?scp=77649113662&partnerID=8YFLogxK

U2 - 10.1161/ATVBAHA.109.200097

DO - 10.1161/ATVBAHA.109.200097

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 20007909

AN - SCOPUS:77649113662

SN - 1079-5642

VL - 30

SP - 533

EP - 535

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

IS - 3

ER -

A specific subset of mouse bone marrow cells has smooth muscle cell differentiation capacity-brief report

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this