A simple method to optimize elution from affinity chromatography

Mordechai Weiss; Zemach Eisenstein

doi:10.1080/01483918708066829

A simple method to optimize elution from affinity chromatography

Mordechai Weiss, Zemach Eisenstein

Clinical Departments

Chaim Sheba Medical Center

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A rapid and convenient screening method to optimize elution fran affinity chranatography is described. An immunoaffinity system employing antibody bound human thyroglobulin (hTg) served as a model. Anti-hTg was complexed to polystyrene microplates adsorbed hTg. Immune complex (IC) dissociation and elution of antigen by various agents was determined by enzyme linked immunosorbent assay (ELISA). The method permits simultaneous monitoring of residual antigenicity and of possible desorption of antigen from its solid support.

Original language	English
Pages (from-to)	2815-2824
Number of pages	10
Journal	Journal of Liquid Chromatography
Volume	10
Issue number	13
DOIs	https://doi.org/10.1080/01483918708066829
State	Published - 1 Oct 1987

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10.1080/01483918708066829

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AB - A rapid and convenient screening method to optimize elution fran affinity chranatography is described. An immunoaffinity system employing antibody bound human thyroglobulin (hTg) served as a model. Anti-hTg was complexed to polystyrene microplates adsorbed hTg. Immune complex (IC) dissociation and elution of antigen by various agents was determined by enzyme linked immunosorbent assay (ELISA). The method permits simultaneous monitoring of residual antigenicity and of possible desorption of antigen from its solid support.

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ER -

A simple method to optimize elution from affinity chromatography

Abstract

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