A simple method for the production of migration inhibitory factor by concanavalin A-stimulated lymphocytes

Edgar Pick, Philip Kotkes

Research output: Contribution to journalArticlepeer-review

Abstract

A simple method for preparing culture supernatants containing macrophage migration inhibitory factor (MIF) by pulse exposure of guinea pig lymphocytes to concanavalin A (Con A), is described. The method is based on the property of originally nonadherent lymphocytes to attach to the flask surface in the presence of Con A. The Con A-induced lymphocyte 'monolayer' can be easily rinsed, allowing the removal of free Con A without repeated, cell damaging centrifugation. The Con A-pulsed lymphocytes are cultured for 24 h in medium and supernatants containing MIF but free of undesired Con A are regularly obtained.

Original languageEnglish
Pages (from-to)141-146
Number of pages6
JournalJournal of Immunological Methods
Volume14
Issue number2
DOIs
StatePublished - Feb 1977

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