TY - JOUR
T1 - A simple method for the production of migration inhibitory factor by concanavalin A-stimulated lymphocytes
AU - Pick, Edgar
AU - Kotkes, Philip
N1 - Funding Information:
This research was supported by grant 5 R01 AI 11194 from the National Institutes of Health, Bethesda, Md., by a grant from the United States-Israel Binational Science Foundation and by a grant from F. Hoffmann-LaRoche & Co. Ltd., Basle, Switzerland. The competent secretarial help of Mrs. Julia Schwartz is greatfully acknowledged.
PY - 1977/2
Y1 - 1977/2
N2 - A simple method for preparing culture supernatants containing macrophage migration inhibitory factor (MIF) by pulse exposure of guinea pig lymphocytes to concanavalin A (Con A), is described. The method is based on the property of originally nonadherent lymphocytes to attach to the flask surface in the presence of Con A. The Con A-induced lymphocyte 'monolayer' can be easily rinsed, allowing the removal of free Con A without repeated, cell damaging centrifugation. The Con A-pulsed lymphocytes are cultured for 24 h in medium and supernatants containing MIF but free of undesired Con A are regularly obtained.
AB - A simple method for preparing culture supernatants containing macrophage migration inhibitory factor (MIF) by pulse exposure of guinea pig lymphocytes to concanavalin A (Con A), is described. The method is based on the property of originally nonadherent lymphocytes to attach to the flask surface in the presence of Con A. The Con A-induced lymphocyte 'monolayer' can be easily rinsed, allowing the removal of free Con A without repeated, cell damaging centrifugation. The Con A-pulsed lymphocytes are cultured for 24 h in medium and supernatants containing MIF but free of undesired Con A are regularly obtained.
UR - http://www.scopus.com/inward/record.url?scp=0017357433&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(77)90004-7
DO - 10.1016/0022-1759(77)90004-7
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AN - SCOPUS:0017357433
SN - 0022-1759
VL - 14
SP - 141
EP - 146
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -