TY - JOUR
T1 - A simple method for identifying the palindromic sequences recognized by restriction endonucleases
T2 - the nucleotide sequence of the AvaII site
AU - Fuchs, Camil
AU - Rosenvold, Eric C.
AU - Honigman, Alik
AU - Szybalski, Waclaw
N1 - Funding Information:
This work was supported by a program-project grant from the National Cancer Institute (CA-07175). We are greatly obliged to Dr. F, Sanger of ~ M.R.C, Cambridge, England and to Dr. S.M. Weissman of Yale University for supplying us with their unpublished data on the sequences of ¢X174 and SV40 DNAs, respectively, The authors are grateful to Dr. Andrew Siegel, Dept. of Statistics and Drs. JanetiVIertz and Bill Sugden of the McArdle Laboratory for helpful discussions. We would also like to thank Ms, Helen Pong for valuable computing assistance.
PY - 1978/9
Y1 - 1978/9
N2 - Tables specifying the frequencies, distances between and positions of all possible tetra-, penta- and hexanucleotide palindromes in øX174 and SV40 viral DNAs were prepared by a computer search of their base sequences. A simple method based on these tables is described for identifying the sequence recognized by any specific restriction endonuclease. The method requires experimental determination of the number and approximate sizes of the fragments obtained by digestion of øX174 RF and SV40 DNAs. Using this method we identified the sequence for the AvaII restriction endonuclease as 5′-GG(TA)CC.
AB - Tables specifying the frequencies, distances between and positions of all possible tetra-, penta- and hexanucleotide palindromes in øX174 and SV40 viral DNAs were prepared by a computer search of their base sequences. A simple method based on these tables is described for identifying the sequence recognized by any specific restriction endonuclease. The method requires experimental determination of the number and approximate sizes of the fragments obtained by digestion of øX174 RF and SV40 DNAs. Using this method we identified the sequence for the AvaII restriction endonuclease as 5′-GG(TA)CC.
UR - http://www.scopus.com/inward/record.url?scp=0018009117&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(78)90011-2
DO - 10.1016/0378-1119(78)90011-2
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0018009117
SN - 0378-1119
VL - 4
SP - 1
EP - 23
JO - Gene
JF - Gene
IS - 1
ER -