A simple method for estimating oral microbial levels

Mel Rosenberg*, Mira Barki, Susana Portnoy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


A simple test is described which enables estimation of oral microbial levels. The test is based on three steps: (1) oral rinsing with ultrahigh-temperature-sterilized milk, (2) expectoration of the sample into a vessel containing methylene blue, and (3) measurement of the time required for colour change at the bottom of the vessel, indicating anaerobiosis. The results correlate highly with microbial counts (colony-forming units, aerobic conditions) in the expectorate. The method may have potential clinical applications as a test for measurement of oral hygiene levels.

Original languageEnglish
Pages (from-to)253-256
Number of pages4
JournalJournal of Microbiological Methods
Issue number4
StatePublished - Jun 1989


  • Method
  • Methylene blue
  • Milk
  • O
  • Oral microorganism


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