TY - JOUR
T1 - A simple method for estimating oral microbial levels
AU - Rosenberg, Mel
AU - Barki, Mira
AU - Portnoy, Susana
PY - 1989/6
Y1 - 1989/6
N2 - A simple test is described which enables estimation of oral microbial levels. The test is based on three steps: (1) oral rinsing with ultrahigh-temperature-sterilized milk, (2) expectoration of the sample into a vessel containing methylene blue, and (3) measurement of the time required for colour change at the bottom of the vessel, indicating anaerobiosis. The results correlate highly with microbial counts (colony-forming units, aerobic conditions) in the expectorate. The method may have potential clinical applications as a test for measurement of oral hygiene levels.
AB - A simple test is described which enables estimation of oral microbial levels. The test is based on three steps: (1) oral rinsing with ultrahigh-temperature-sterilized milk, (2) expectoration of the sample into a vessel containing methylene blue, and (3) measurement of the time required for colour change at the bottom of the vessel, indicating anaerobiosis. The results correlate highly with microbial counts (colony-forming units, aerobic conditions) in the expectorate. The method may have potential clinical applications as a test for measurement of oral hygiene levels.
KW - Method
KW - Methylene blue
KW - Milk
KW - O
KW - Oral microorganism
UR - http://www.scopus.com/inward/record.url?scp=0024386689&partnerID=8YFLogxK
U2 - 10.1016/0167-7012(89)90067-5
DO - 10.1016/0167-7012(89)90067-5
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AN - SCOPUS:0024386689
SN - 0167-7012
VL - 9
SP - 253
EP - 256
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 4
ER -