A simple method for estimating oral microbial levels

Mel Rosenberg; Mira Barki; Susana Portnoy

doi:10.1016/0167-7012(89)90067-5

A simple method for estimating oral microbial levels

Mel Rosenberg^*, Mira Barki, Susana Portnoy

^*Corresponding author for this work

Clinical Microbiology and Immunology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A simple test is described which enables estimation of oral microbial levels. The test is based on three steps: (1) oral rinsing with ultrahigh-temperature-sterilized milk, (2) expectoration of the sample into a vessel containing methylene blue, and (3) measurement of the time required for colour change at the bottom of the vessel, indicating anaerobiosis. The results correlate highly with microbial counts (colony-forming units, aerobic conditions) in the expectorate. The method may have potential clinical applications as a test for measurement of oral hygiene levels.

Original language	English
Pages (from-to)	253-256
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	9
Issue number	4
DOIs	https://doi.org/10.1016/0167-7012(89)90067-5
State	Published - Jun 1989

Keywords

Method
Methylene blue
Milk
O
Oral microorganism

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10.1016/0167-7012(89)90067-5

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AB - A simple test is described which enables estimation of oral microbial levels. The test is based on three steps: (1) oral rinsing with ultrahigh-temperature-sterilized milk, (2) expectoration of the sample into a vessel containing methylene blue, and (3) measurement of the time required for colour change at the bottom of the vessel, indicating anaerobiosis. The results correlate highly with microbial counts (colony-forming units, aerobic conditions) in the expectorate. The method may have potential clinical applications as a test for measurement of oral hygiene levels.

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KW - Methylene blue

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A simple method for estimating oral microbial levels

Abstract

Keywords

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