A simple FRET-based modular design for diagnostic probes

Orit Redy, Einat Kisin-Finfer, Eran Sella, Doron Shabat*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


In recent years, there has been a massive effort to develop molecular probes with optical modes of action. Probes generally produce detectable signals based on changes in fluorescence properties. Here, we demonstrate the potential of self-immolative molecular adaptors as a platform for Turn-On probes based on the FRET technique. The probe is equipped with identical fluorophore pairs or a fluorophore/quencher FRET pair and a triggering substrate. Upon reaction of the analyte of interest with the triggering substrate, the self-immolative adaptor spontaneously releases the two dye molecules to break off the FRET effect. As a result, a new measurable fluorescent signal is generated. The fluorescence obtained can be used to quantify the analyte. The modular structure of the probe design will allow the preparation of various chemical probes based on the FRET activation technique.

Original languageEnglish
Pages (from-to)710-715
Number of pages6
JournalOrganic and Biomolecular Chemistry
Issue number4
StatePublished - 28 Jan 2012


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