A second isoform of gonadotropin-releasing hormone is present in the brain of human and rodents

Alon Chen, Dror Yahalom, Nurit Ben-Aroya, Ella Kaganovsky, Eli Okon, Yitzhak Koch*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Gonadotropin-releasing hormone-I (GnRH-I), present in the mammalian hypothalamus, regulates reproduction. In this study we demonstrate, for the first time, that an additional isoform of GnRH, [His5, Trp7, Tyr8] GnRH-I (GnRH-II) is present in the brain of the mouse, rat and human. Human and rat brain extracts contain two isoforms of GnRH, GnRH-I and GnRH-II, which exhibited identical chromatographic properties to the respective synthetic peptides, in high performance liquid chromatography. Using immunohistochemical techniques we have found that GnRH-II is present in neuronal cells that are localized mainly in the periaqueductal area as well as in the oculomotor and red nuclei of the midbrain. It is of interest to note that in the hypogonadal mouse, although the GnRH-I gene is deleted, GnRH-II is present. Substantial concentrations of GnRH-II are also present in the hypothalamus and stored in the human pituitary stalk or in the mouse median eminence. By using reverse transcription (RT)-PCR we have also found that while GnRH-II is not expressed in the cerebellum, it is expressed in all three structures of the brain stem: midbrain, pons and medulla oblongata. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)199-203
Number of pages5
JournalFEBS Letters
Issue number2-3
StatePublished - 18 Sep 1998
Externally publishedYes


FundersFunder number
Grodetsky Center for Research of Higher Brain Functions of the Weizmann Institute of Science


    • GnRH-II in human and rodents
    • GnRH/LHRH isoform
    • Neuropeptide


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