TY - JOUR
T1 - A scheme for sequencing large DNA molecules by identifying local nuclear-induced effects
AU - Kelson, Itzhak
AU - Nussinov, Shmuel
PY - 1994/7/19
Y1 - 1994/7/19
N2 - An experimental scheme for sequencing large DNA molecules is proposed where DNA strands are replicated, with all nucleotides of a given kind marked with radioactive 32P. The marked strands are affixed to an appropriate substrate and are kept until most 32P atoms decay. The local damage caused by the decay is expected to allow the identification of the sites occupied by that particular nucleotide, using atomic scale microscopy (scanning tunneling or atomic force microscopy). Quantitative aspects and methodological considerations associated with the proposed scheme are discussed.
AB - An experimental scheme for sequencing large DNA molecules is proposed where DNA strands are replicated, with all nucleotides of a given kind marked with radioactive 32P. The marked strands are affixed to an appropriate substrate and are kept until most 32P atoms decay. The local damage caused by the decay is expected to allow the identification of the sites occupied by that particular nucleotide, using atomic scale microscopy (scanning tunneling or atomic force microscopy). Quantitative aspects and methodological considerations associated with the proposed scheme are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0028283133&partnerID=8YFLogxK
U2 - 10.1073/pnas.91.15.6963
DO - 10.1073/pnas.91.15.6963
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AN - SCOPUS:0028283133
SN - 0027-8424
VL - 91
SP - 6963
EP - 6966
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 15
ER -