A rat brain cDNA encoding the neurotransmitter transporter with an unusual structure

Qing Rong Liu, Sreekala Mandiyan, Beatriz López-Corcuera, Hannah Nelson, Nathan Nelson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

A rat cDNA clone encoding the novel membrane protein of the neurotransmitter transporters family was cloned and sequenced. The cDNA was identified as a transcript of the gene NTT4 of which a partial genomic clone was previously sequenced. Alignment of the amino acid sequence of NTT4 with other members of the neurotransmitter transport family revealed a marked deviattion from the conserved structure of all other members of the family. The largest extracellular loop with a potential glycosylation site was identified between membrane segments 7 and 8. The protein retains the common glycosylated loop between transmembrane helices 3 and 4 in all members of the family. The transcript of NTT4 was found exclusively in the central nervous system and is more abundant in the cerebellum and the cerebral cortex.

Original languageEnglish
Pages (from-to)114-118
Number of pages5
JournalFEBS Letters
Volume315
Issue number2
DOIs
StatePublished - 4 Jan 1993
Externally publishedYes

Keywords

  • Central nervous system
  • Molecular cloning
  • Neurotransmitter transporter

Fingerprint

Dive into the research topics of 'A rat brain cDNA encoding the neurotransmitter transporter with an unusual structure'. Together they form a unique fingerprint.

Cite this