A Rapid Fluorescence-Based Screen to Identify Regulators and Components of Interbacterial Competition Mechanisms in Bacteria

Daniel Tchelet, Dor Salomon

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Contact-dependent antibacterial mechanisms enhance bacterial fitness as they enable bacteria to outcompete their rivals and thrive in diverse environments. Such systems also allow pathogenic bacteria to establish a niche inside a host, where they must compete with commensal microflora. In many cases, antibacterial systems are tightly regulated by complex sensor and signal transduction networks. Deciphering these regulatory networks, as well as identifying functional components of antibacterial mechanisms, are valuable objectives since essential regulators and components present possible targets for developing antivirulence therapies. Here we describe Bacterial Competition Fluorescence (BaCoF), a methodology that relies on a fluorescence signal to determine the outcome of bacterial competitions. This methodology enables screening of mutant libraries to identify genes that are essential for activating a contact-dependent antibacterial system of interest. Thus, this methodology can be applied to reveal essential regulators and components of antibacterial systems in bacterial pathogens.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages11-24
Number of pages14
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2427
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Antibacterial toxin
  • Bacterial competition
  • Contact-dependent
  • Regulation
  • T6SS
  • Transposon mutagenesis

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