TY - JOUR
T1 - A radioimmunoassay with monoclonal antibodies for the detection of antigenic cell-free Fc receptor
AU - Ran, Maya
AU - Dux, Zahava
AU - Anavi, Romema
AU - Smorodinsky, Nechama I.
AU - Witz, I. P.
PY - 1984/3/30
Y1 - 1984/3/30
N2 - Making use of 125I-labelled monoclonal rat antibodies against mouse Fc receptor we have developed a solid-phase radioimmunoassay for cell-free antigenic mouse Fc receptor (FcR). An 80% acetone solution was used for fixation of relatively large amounts of soluble proteins on PVC microtiter plates. As a result of this treatment FcR practically lost its activity to bind the Fc portion of an IgG molecule but retained its antigenicity, the binding of specific anti-FcR (aFcR) antibody being increased following acetone fixation. Concentrations of cell-free FcR in NP-40 extracts of FcR-expressing cells were calculated from the linear part of a standard curve and expressed in units of antigenic activity, 1 unit being the amount of antigenic FcR capable of binding 1 μg of 125I-aFcR. The method may be used for detecting cell-free FcR as a minor constituent in a mixture of proteins.
AB - Making use of 125I-labelled monoclonal rat antibodies against mouse Fc receptor we have developed a solid-phase radioimmunoassay for cell-free antigenic mouse Fc receptor (FcR). An 80% acetone solution was used for fixation of relatively large amounts of soluble proteins on PVC microtiter plates. As a result of this treatment FcR practically lost its activity to bind the Fc portion of an IgG molecule but retained its antigenicity, the binding of specific anti-FcR (aFcR) antibody being increased following acetone fixation. Concentrations of cell-free FcR in NP-40 extracts of FcR-expressing cells were calculated from the linear part of a standard curve and expressed in units of antigenic activity, 1 unit being the amount of antigenic FcR capable of binding 1 μg of 125I-aFcR. The method may be used for detecting cell-free FcR as a minor constituent in a mixture of proteins.
KW - Fc receptor assay
KW - cell-free Fc receptor
KW - monoclonal anti-Fc antibody
UR - http://www.scopus.com/inward/record.url?scp=0021258838&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(84)90158-3
DO - 10.1016/0022-1759(84)90158-3
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0021258838
SN - 0022-1759
VL - 68
SP - 275
EP - 284
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -