A quantitative gibberellin signaling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem

Bihai Shi, Amelia Felipo-Benavent, Guillaume Cerutti, Carlos Galvan-Ampudia, Lucas Jilli, Geraldine Brunoud, Jérome Mutterer, Elody Vallet, Lali Sakvarelidze-Achard, Jean Michel Davière, Alejandro Navarro-Galiano, Ankit Walia, Shani Lazary, Jonathan Legrand, Roy Weinstain, Alexander M. Jones, Salomé Prat, Patrick Achard*, Teva Vernoux*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remains mostly unknown. Here, we developed a ratiometric GA signaling biosensor by engineering one of the DELLA proteins, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this degradation-based biosensor accurately reports on cellular changes in GA levels and perception during development. We used this biosensor to map GA signaling activity in the SAM. We show that high GA signaling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organization of internodes, thus contributing to internode specification in the SAM.

Original languageEnglish
Article number3895
JournalNature Communications
Volume15
Issue number1
DOIs
StatePublished - Dec 2024

Funding

FundersFunder number
Institut de Biologie Moléculaire des Plantes
SFR Biosciences
National Agricultural Science and Technology Center
European Research Council
Laboratoire Reproduction et Développement des Plantes
ANR-16-CE13-0014
Guangdong Laboratory for Lingnan Modern AgricultureNG2021001
Israel Science Foundation1057/21
Israel Science Foundation

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