TY - JOUR
T1 - A quantitative gibberellin signaling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem
AU - Shi, Bihai
AU - Felipo-Benavent, Amelia
AU - Cerutti, Guillaume
AU - Galvan-Ampudia, Carlos
AU - Jilli, Lucas
AU - Brunoud, Geraldine
AU - Mutterer, Jérome
AU - Vallet, Elody
AU - Sakvarelidze-Achard, Lali
AU - Davière, Jean Michel
AU - Navarro-Galiano, Alejandro
AU - Walia, Ankit
AU - Lazary, Shani
AU - Legrand, Jonathan
AU - Weinstain, Roy
AU - Jones, Alexander M.
AU - Prat, Salomé
AU - Achard, Patrick
AU - Vernoux, Teva
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/12
Y1 - 2024/12
N2 - Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remains mostly unknown. Here, we developed a ratiometric GA signaling biosensor by engineering one of the DELLA proteins, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this degradation-based biosensor accurately reports on cellular changes in GA levels and perception during development. We used this biosensor to map GA signaling activity in the SAM. We show that high GA signaling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organization of internodes, thus contributing to internode specification in the SAM.
AB - Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remains mostly unknown. Here, we developed a ratiometric GA signaling biosensor by engineering one of the DELLA proteins, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this degradation-based biosensor accurately reports on cellular changes in GA levels and perception during development. We used this biosensor to map GA signaling activity in the SAM. We show that high GA signaling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organization of internodes, thus contributing to internode specification in the SAM.
UR - http://www.scopus.com/inward/record.url?scp=85192604108&partnerID=8YFLogxK
U2 - 10.1038/s41467-024-48116-4
DO - 10.1038/s41467-024-48116-4
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C2 - 38719832
AN - SCOPUS:85192604108
SN - 2041-1723
VL - 15
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 3895
ER -