A Protocol for Simple, Rapid, and Direct Detection of SARS-CoV-2 from clinical samples, using Reverse Transcribed Loop-Mediated Isothermal Amplification (RT-LAMP)

Rawi Naddaf, Nadav Ben-Assa, Tal Gefen, Tal Capucha, Haitham Hajjo, Noa Mandelbaum, Lilach Elbaum, Shai Kaplan, Assaf Rotem, Michal Chowers, Moran Szwarcwort-Cohen, Mical Paul, Naama Geva-Zatorsky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

SARS-CoV-2 has quickly spread all around the globe causing illness and wide damages. Most countries were unprepared for such a rapid spread and crisis. This led to various strategies for effective control of the new pandemic. A key aspect in all countries was to effectively test the population for the virus. Most countries chose a lockdown strategy in which many workplaces and activities are completely closed, leading to substantial economy costs. Here, we present a protocol we recently developed that allows rapid and simple detection of SARS-CoV-2 for the large population, eliminating costs and involvement of professional teams and laboratories. This protocol is based on Reverse Transcribed Loop-Mediated Isothermal Amplification (RT-LAMP). We tested this protocol directly on patient samples, both nasal and throat clinical swabs as well as saliva. Notably, this protocol is simple, cheap and can be easily applied to other pathogens as well.

Original languageEnglish
Article numbere3789
JournalBio-protocol
Volume10
Issue number20
DOIs
StatePublished - 2020

Funding

FundersFunder number
Technion Integrated Cancer Center
Canadian Institute for Advanced Research
Technion-Israel Institute of Technology

    Keywords

    • Colorimetric
    • Covid-19
    • Isothermal
    • Pandemic
    • RT-LAMP
    • Rapid molecular Detection
    • SARS-CoV-2
    • Saliva

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