A protein of neutrophil granules interferes with activation of NADPH oxidase in a cell-free system

Irit Aviram*, Anat Faber

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A soluble extract of neutrophil granules interfered with activation of the NADPH oxidase in a cell-free system. The extract had no effect on superoxide production by preactivated enzyme. The inhibitory activity was retained during dialysis and was lost upon exposure to proteinase K indicating that the active substance was a protein. The inhibitor exhibited a high stability at elevated temperatures. Chromatography of granules extract on ion exchangers implied that the inhibitor was a positively charged protein eluting from S Sepharose cation exchanger above 0.4M concentration of NaCl.

Original languageEnglish
Pages (from-to)198-202
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume169
Issue number1
DOIs
StatePublished - 31 May 1990

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