A procedure for amino acid sequencing in internal regions of proteins

Itai Benhar, Hanna Engelberg-Kulka*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


We describe a novel procedure for determining the amino acid (aa) sequence of the internal regions of proteins. This procedure has been implemented by directly determining the sequence of aa 65-75 of the product of the trpR gene of Escherichia coli, the trp repressor. This method is based on the insertion of the cleavage site of a specific protease (factor Xa) into the protein immediately before the region to be sequenced by Edman degradation. The simplicity of the procedure makes it appealing for studies of protein structure-function relationships, and of the expression of genetic information. The method is particularly useful when there is ambiguity concerning the co-linearity of the aa and nucleotide sequences.

Original languageEnglish
Pages (from-to)79-82
Number of pages4
Issue number1
StatePublished - 15 Jul 1991
Externally publishedYes


  • Amino acid sequence
  • Edman degradation
  • in vitro mutagenesis
  • specific proteolytic cleavage
  • trp repressor


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