A precision technology for controlling protein adsorption and cell adhesion in biomems

Y. V. Pan; Y. Hanein; D. Leach-Scampavia; K. F. Böhringer; B. D. Ratner; D. D. Denton

A precision technology for controlling protein adsorption and cell adhesion in biomems

Y. V. Pan^*
, Y. Hanein
, D. Leach-Scampavia
, K. F. Böhringer
, B. D. Ratner
, D. D. Denton

^*Corresponding author for this work

University of Washington

Research output: Contribution to conference › Paper › peer-review

9 Scopus citations

Abstract

A surface coating technique is investigated to enhance device biocompatibility by eliminating bio-fouling, the strong but non-specific affinity of proteins and cells to attach to surfaces. This coating is a conformal, thin poly(ethylene glycol)-like film deposited in a glow discharge of tetraglyme. Substrates with different chemistries are successfully modified, and exhibit ultralow protein adsorption and cell attachment with the coating. This "stealth" or "non-fouling" coating can also be faithfully patterned using standard photolithography processes. The interaction of proteins and cells with patterned surfaces is limited only to the protein-adhesive domains, thus creating heterogeneous patterns of proteins and cell cultures on the surface. The potential benefits of our technique to applications such as cell-based assays and micro-electrodes are discussed.

Original language	English
Pages	435-438
Number of pages	4
State	Published - 2001
Externally published	Yes
Event	14th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2001) - Interlaken, Switzerland Duration: 21 Jan 2001 → 25 Jan 2001

Conference

Conference	14th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2001)
Country/Territory	Switzerland
City	Interlaken
Period	21/01/01 → 25/01/01

Cite this

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T1 - A precision technology for controlling protein adsorption and cell adhesion in biomems

AU - Pan, Y. V.

AU - Hanein, Y.

AU - Leach-Scampavia, D.

AU - Böhringer, K. F.

AU - Ratner, B. D.

AU - Denton, D. D.

PY - 2001

Y1 - 2001

N2 - A surface coating technique is investigated to enhance device biocompatibility by eliminating bio-fouling, the strong but non-specific affinity of proteins and cells to attach to surfaces. This coating is a conformal, thin poly(ethylene glycol)-like film deposited in a glow discharge of tetraglyme. Substrates with different chemistries are successfully modified, and exhibit ultralow protein adsorption and cell attachment with the coating. This "stealth" or "non-fouling" coating can also be faithfully patterned using standard photolithography processes. The interaction of proteins and cells with patterned surfaces is limited only to the protein-adhesive domains, thus creating heterogeneous patterns of proteins and cell cultures on the surface. The potential benefits of our technique to applications such as cell-based assays and micro-electrodes are discussed.

AB - A surface coating technique is investigated to enhance device biocompatibility by eliminating bio-fouling, the strong but non-specific affinity of proteins and cells to attach to surfaces. This coating is a conformal, thin poly(ethylene glycol)-like film deposited in a glow discharge of tetraglyme. Substrates with different chemistries are successfully modified, and exhibit ultralow protein adsorption and cell attachment with the coating. This "stealth" or "non-fouling" coating can also be faithfully patterned using standard photolithography processes. The interaction of proteins and cells with patterned surfaces is limited only to the protein-adhesive domains, thus creating heterogeneous patterns of proteins and cell cultures on the surface. The potential benefits of our technique to applications such as cell-based assays and micro-electrodes are discussed.

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T2 - 14th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2001)

Y2 - 21 January 2001 through 25 January 2001

ER -

A precision technology for controlling protein adsorption and cell adhesion in biomems

Abstract

Conference

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