@article{65c00a8498694f6996c1ba2e113d5a8d,
title = "A possible role for cysteine residues in the fidelity of DNA synthesis exhibited by the reverse transcriptases of human immunodeficiency viruses type 1 and type 2",
abstract = "HIV reverse transcriptases (RTs) have few cysteine residues relative to other RTs and retain their DNA polymerization functions following chemical modification by thiol-specific reagents. The functional role of the cysteines in the fidelity of the DNA-dependent DNA synthesis of HIV RTs has been addressed by chemical modification of the wild-type enzymes in combination with the analysis of an enzymatically active mutant HIV-1 RT in which all cysteines were modified to serines. We have observed an increase in 3′-terminal mispair extension efficiency exhibited by chemically modified HIV-1 and HIV-2 RTs. The possible involvement of cysteine residues was further substantiated using the cysteine-free mutant HIV-1 RT that displays an increased efficiency of mispair extension. These results provide evidence for a possible role of cysteine residues in the fidelity of DNA synthesis catalyzed by HIV RTs.",
keywords = "Cysteine, DNA synthesis, Fidelity, HIV, RT",
author = "Mary Bakhanashvili and Amnon Hizi",
note = "Funding Information: purified recombinant reverse transcriptases, and to S. Loya and M, Shaharabany for helpful discussions and to L. Newstead for typing the manuscript, This research was supported by Grant NO Ai27035 from the National Institute of Allergy and Infectious Diseases.",
year = "1992",
month = jun,
day = "15",
doi = "10.1016/0014-5793(92)80640-3",
language = "אנגלית",
volume = "304",
pages = "289--293",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "2-3",
}