A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking

Pablo S. Aguilar, Florian Fröhlich, Michael Rehman, Mike Shales, Igor Ulitsky, Agustina Olivera-Couto, Hannes Braberg, Ron Shamir, Peter Walter, Matthias Mann, Christer S. Ejsing, Nevan J. Krogan, Tobias C. Walther

Research output: Contribution to journalArticlepeer-review


The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.

Original languageEnglish
Pages (from-to)901-908
Number of pages8
JournalNature Structural and Molecular Biology
Issue number7
StatePublished - Jul 2010


FundersFunder number
Estrategia Nacional de Innovación
International Human Frontier Science Program
National Institutes of Health
National Institute of General Medical SciencesR01GM084448
Agencia Nacional de Investigación e Innovación
Human Frontier Science Program
Deutsche Forschungsgemeinschaft
German-Israeli Foundation for Scientific Research and Development
Israel Science Foundation802/08
Tel Aviv University


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