A pilot trial of tumor lysate-loaded dendritic cells for the treatment of metastatic renal cell carcinoma

Barbara J. Gitlitz, Arie S. Belldegrun, Amnon Zisman, Debby H. Chao, Allan J. Pantuck, Andreas Hinkel, Peter Mulders, Nancy Moldawer, Cho Lea Tso, Robert A. Figlin

Research output: Contribution to journalArticlepeer-review

Abstract

Cultured tumor lysate-loaded dendritic cells (TuLy-DC) have been demonstrated in vitro to stimulate potent immune modulations and generate significant antitumor response. We report the results of a pilot trial of TuLy-DC vaccine for patients with metastatic renal cell carcinoma (mRCC). Fourteen mRCC patients underwent nephrectomy to obtain autologous TuLy prepared by subjecting tumor cells to 3 freeze/thaw cycles. Dendritic cells were generated from peripheral blood CD14+ precursors cultured in the presence of GM-CSF, IL-4, and 10% autologous serum. Patients received one vaccination of TuLy alone as an immunologic control, followed by 3 weekly vaccinations of DC-TuLy injected intradermally in the midaxillary region. Peripheral blood lymphocytes were collected before and after weekly vaccines and were assessed for changes in phenotype, cytotoxicity, and cytokine profile. The TuLy-DC vaccine was successfully prepared and administered to 12 patients, whereas 2 patients did not receive vaccine treatment due to declines in postoperative performance status. The vaccines were well tolerated, with only grade 1 toxicities noted. One patient had a partial response to treatment that did not correspond to any significant change in immunologic profile. This pilot trial demonstrated both the safety and feasibility of reliably preparing a DC-based vaccine for mRCC patients. Our data suggest that autologous TuLy-DC vaccines generate only limited clinical response. Further clinical studies are needed to identify the most potent treatment regimen that can consistently mediate an antitumor immune response in vivo.

Original languageEnglish
Pages (from-to)412-419
Number of pages8
JournalJournal of Immunotherapy
Volume26
Issue number5
DOIs
StatePublished - 2003
Externally publishedYes

Keywords

  • Antitumor response
  • Immune modulation
  • Kidney cancer
  • Vaccine

Fingerprint

Dive into the research topics of 'A pilot trial of tumor lysate-loaded dendritic cells for the treatment of metastatic renal cell carcinoma'. Together they form a unique fingerprint.

Cite this