A physical map across chromosome 11q22-q23 containing the major locus for ataxia telangiectasia

Helen J. Ambrose, Philip J. Byrd, Carmel M. McConville, Paul R. Cooper, Tanja Stankovic, John H. Riley, Yosef Shiloh, James O. McNamara, Toshiyuki Fukao, A. Malcolm R. Taylor*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We have constructed a long-range physical map for 12 markers, including genes for GRIA4, IL1BC, and ACAT, across 9 Mb of chromosome 11q22-q23 in the region of the major locus for ataxia-telangiectasia (A-T). The markers fall into proximal and distal groups with respect to the centromere. We have linked the proximal and distal groups by hybridization to a 2.7-Mb NotI fragment and a 4.6-Mb MluI fragment. The following locus order was obtained: centromere-CJ52.75-J12.1C2-Y11B11R-IL1BC-hbcDNA-GRIA4-CJ52.3-Y11B29L-ACA T-CJ52.193-J12.8-Y11B06R-telomere. We show that hbcDNA/GRIA4 and CJ52.3 are very closely linked to each end, respectively, of the 2.7-Mb NotI fragment, thereby fixing the position of the complete contig. Our results indicate that the gene for A-T is flanked by the markers GRIA4 and J12.8, which are no more than 3 Mb apart, on a 4.6-Mb Mlu I fragment. The physical map allows rapid positioning of markers, and this will facilitate the construction of a YAC contig across the region.

Original languageEnglish
Pages (from-to)612-619
Number of pages8
JournalGenomics
Volume21
Issue number3
DOIs
StatePublished - Jun 1994

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