TY - JOUR
T1 - A one-step, separation-free amperometric enzyme immunosensor
AU - Ivnitski, D.
AU - Rishpon, J.
N1 - Funding Information:
The authors wish to thank the European Union--AVICENNE Program for financial support.
PY - 1996
Y1 - 1996
N2 - A new one-step, separation-free, amperometric enzyme immunosensor is described. The sensor consists of an antibody electrode that is low cost, disposable, and operates without washing or separation steps. The immunosensor combines the following signal-amplification systems: enzyme-channeling immunoassay; accumulation of the redox mediators (I2/I-); cyclic regeneration of an enzyme (peroxidase) substrate at the (polyethylenimine) polymer/electrode interface; and control of the hydrodynamic conditions at the interface of the antibody electrode. The immunological reactions were monitored electrochemically in situ, and the binding curves were directly visualized on a computer screen. The complete immunoassay can be performed in 5-20 min depending on the complexity of the immunological reactions. Model systems using rabbit IgG and human luteinizing hormone (hLH) in a 'sandwich' immunoassay revealed that the immunosensor can detect concentrations of hLH in human serum as low as 1 ng ml-1.
AB - A new one-step, separation-free, amperometric enzyme immunosensor is described. The sensor consists of an antibody electrode that is low cost, disposable, and operates without washing or separation steps. The immunosensor combines the following signal-amplification systems: enzyme-channeling immunoassay; accumulation of the redox mediators (I2/I-); cyclic regeneration of an enzyme (peroxidase) substrate at the (polyethylenimine) polymer/electrode interface; and control of the hydrodynamic conditions at the interface of the antibody electrode. The immunological reactions were monitored electrochemically in situ, and the binding curves were directly visualized on a computer screen. The complete immunoassay can be performed in 5-20 min depending on the complexity of the immunological reactions. Model systems using rabbit IgG and human luteinizing hormone (hLH) in a 'sandwich' immunoassay revealed that the immunosensor can detect concentrations of hLH in human serum as low as 1 ng ml-1.
KW - Amperometry
KW - Disposable electrode
KW - Enzyme immunoassay
KW - Immunosensor
KW - Signal-amplification systems
UR - http://www.scopus.com/inward/record.url?scp=0030056929&partnerID=8YFLogxK
U2 - 10.1016/0956-5663(96)82736-9
DO - 10.1016/0956-5663(96)82736-9
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AN - SCOPUS:0030056929
SN - 0956-5663
VL - 11
SP - 409
EP - 417
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 4
ER -