A NusG-like transcription anti-terminator is involved in the biosynthesis of the polyketide antibiotic TA of Myxococcus xanthus

Yossi Paitan; Elisha Orr; Eliora Z. Ron; Eugene Rosenberg

doi:10.1016/S0378-1097(98)00542-4

A NusG-like transcription anti-terminator is involved in the biosynthesis of the polyketide antibiotic TA of Myxococcus xanthus

Yossi Paitan, Elisha Orr, Eliora Z. Ron, Eugene Rosenberg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The antibiotic TA of Myxococcus xanthus is synthesized through a type I polyketide synthase mechanism. Previous studies have indicated that several genes essential for TA production are clustered within a 40-kb region and are transcriptionally co-regulated. In this study, we report the genetic analysis of the first gene in the TA gene cluster, identified as a NusG-like transcription anti-terminator. Functional analysis of this NusG-like anti-terminator gene by specific gene disruption confirms that it is essential for TA production but not for normal growth and development. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	221-227
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	170
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(98)00542-4
State	Published - 1 Jan 1999

Keywords

Antibiotic biosynthesis
Myxococcus xanthus
NusG
Polyketide
Transcription anti-termination
Type I polyketide synthase

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10.1016/S0378-1097(98)00542-4

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title = "A NusG-like transcription anti-terminator is involved in the biosynthesis of the polyketide antibiotic TA of Myxococcus xanthus",

abstract = "The antibiotic TA of Myxococcus xanthus is synthesized through a type I polyketide synthase mechanism. Previous studies have indicated that several genes essential for TA production are clustered within a 40-kb region and are transcriptionally co-regulated. In this study, we report the genetic analysis of the first gene in the TA gene cluster, identified as a NusG-like transcription anti-terminator. Functional analysis of this NusG-like anti-terminator gene by specific gene disruption confirms that it is essential for TA production but not for normal growth and development. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Antibiotic biosynthesis, Myxococcus xanthus, NusG, Polyketide, Transcription anti-termination, Type I polyketide synthase",

author = "Yossi Paitan and Elisha Orr and Ron, {Eliora Z.} and Eugene Rosenberg",

note = "Funding Information: We thank Dr. Steven G. Sedgwick for providing the tagged Tn 1000 system. This work was supported in part by the Pasha Gol Chair for Applied Microbiology (E.R.), the Morris and Manja Leigh Chair for Biophysics and Biotechnology (E.Z.R.), a FEMS Research fellowship awarded to Y.P., Cancer Research Campaign (SP1937/0301) and Wellcome Trust (038060/Z/93/Z) grants to E.O. ",

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doi = "10.1016/S0378-1097(98)00542-4",

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AU - Ron, Eliora Z.

AU - Rosenberg, Eugene

N1 - Funding Information: We thank Dr. Steven G. Sedgwick for providing the tagged Tn 1000 system. This work was supported in part by the Pasha Gol Chair for Applied Microbiology (E.R.), the Morris and Manja Leigh Chair for Biophysics and Biotechnology (E.Z.R.), a FEMS Research fellowship awarded to Y.P., Cancer Research Campaign (SP1937/0301) and Wellcome Trust (038060/Z/93/Z) grants to E.O.

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N2 - The antibiotic TA of Myxococcus xanthus is synthesized through a type I polyketide synthase mechanism. Previous studies have indicated that several genes essential for TA production are clustered within a 40-kb region and are transcriptionally co-regulated. In this study, we report the genetic analysis of the first gene in the TA gene cluster, identified as a NusG-like transcription anti-terminator. Functional analysis of this NusG-like anti-terminator gene by specific gene disruption confirms that it is essential for TA production but not for normal growth and development. Copyright (C) 1999 Elsevier Science B.V.

AB - The antibiotic TA of Myxococcus xanthus is synthesized through a type I polyketide synthase mechanism. Previous studies have indicated that several genes essential for TA production are clustered within a 40-kb region and are transcriptionally co-regulated. In this study, we report the genetic analysis of the first gene in the TA gene cluster, identified as a NusG-like transcription anti-terminator. Functional analysis of this NusG-like anti-terminator gene by specific gene disruption confirms that it is essential for TA production but not for normal growth and development. Copyright (C) 1999 Elsevier Science B.V.

KW - Antibiotic biosynthesis

KW - Myxococcus xanthus

KW - NusG

KW - Polyketide

KW - Transcription anti-termination

KW - Type I polyketide synthase

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A NusG-like transcription anti-terminator is involved in the biosynthesis of the polyketide antibiotic TA of Myxococcus xanthus

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