A novel U1/U5 interaction indicates proximity between U1 and U5 snRNAs during an early step of mRNA splicing

Gil Ast*, Alan M. Weiner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The five spliceosomal snRNAs (U1, U2, U4, U5, and U6) undergo an ordered sequence of conformational changes as mRNA splicing progresses. We have shown that an antisense RNA oligonucleotide complementary to U5 snRNA induces a novel U1/U4/U5 complex that may be a transitional stage in the displacement of U1 from the 5' splice site by U5. Here we identify a novel site-specific crosslink between the 5' end of U1 and the invariant loop of U5 snRNA. This crosslink can be induced in nuclear extract by an antisense oligonucleotide directed against U5 snRNA, but can also be detected during an early step of the splicing reaction in the absence of oligonucleotide. Our data indicate proximity between U1 and U5 snRNPs before the first catalytic step of splicing, and may suggest that U1 helps to direct U5 to the 5' splice site.

Original languageEnglish
Pages (from-to)371-381
Number of pages11
JournalRNA
Volume3
Issue number4
StatePublished - Apr 1997
Externally publishedYes

Funding

FundersFunder number
National Institute of General Medical SciencesR01GM031073

    Keywords

    • antisense oligonucleotide
    • pre-mRNA splicing
    • psoralen crosslink

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