A novel T-DNA integration in rice involving two interchromosomal translocations

Bharat Bhusan Majhi, Jasmine M. Shah, Karuppannan Veluthambi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Key message: A male sterile transgenic rice plant TC-19 harboured a novel T-DNA integration in chromosome 8 with two interchromosomal translocations of 6.55 kb chromosome 3 and 29.8 kb chromosome 9 segments. We report a complex Agrobacterium T-DNA integration in rice (Oryza sativa) associated with two interchromosomal translocations. The T-DNA-tagged rice mutant TC-19, which harboured a single copy of the T-DNA, displayed male sterile phenotype in the homozygous condition. Analysis of the junctions between the T-DNA ends and the rice genome by genome walking showed that the right border is flanked by a chromosome 3 sequence and the left border is flanked by a chromosome 9 sequence. Upon further walking on chromosome 3, a chromosome 3/chromosome 8 fusion was detected. Genome walking from the opposite end of the chromosome 8 break point revealed a chromosome 8/chromosome 9 fusion. Our findings revealed that the T-DNA, together with a 6.55-kb region of chromosome 3 and a 29.8-kb region of chromosome 9, was translocated to chromosome 8. Southern blot analysis of the homozygous TC-19 mutant revealed that the native sequences of chromosome 3 and 9 were restored but the disruption of chromosome 8 in the first intron of the gene Os08g0152500 was not restored. The integration of the complex T-DNA in chromosome 8 caused male sterility.

Original languageEnglish
Pages (from-to)929-944
Number of pages16
JournalPlant Cell Reports
Issue number6
StatePublished - Jun 2014
Externally publishedYes


  • Agrobacterium
  • Genome walking
  • Interchromosomal translocation
  • Male sterility
  • Rice
  • T-DNA integration


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