A Novel peptide prevents death in enriched neuronal cultures

Ilona Zemlyak, Sharon Furman, Douglas E. Brenneman, Illana Gozes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We have recently cloned a novel protein (activity-dependent neuroprotective protein, ADNP) containing an 8-amino-acid, femtomolar-acting peptide, NAPVSIPQ (NAP). Here we show, for the first time, that NAP exerted a protective effect on glia-depleted neurons in culture. The number of surviving neurons was assessed in cerebral cortical cultures derived from newborn rats. In these cultures, a 24-h treatment with the β-amyloid peptide (the Alzheimer's disease associated toxin) induced a 30-40% reduction in neuronal survival that was prevented by NAP (10-13-10-11 M). Maximal survival was achieved at NAP concentrations of 10-12 M. In a second set of experiments, a 5-day incubation period, with NAP added once (at the beginning of the incubation period) exhibited maximal protection at 10-10 M NAP. In a third set of experiments, a 10-min period of glucose deprivation resulted in a 30-40% neuronal death that was prevented by a 24-h incubation with NAP. Glucose deprivation coupled with β-amyloid treatment did not increase neuronal death, suggesting a common pathway. We thus conclude, that NAP can prevent neurotoxicity associated with direct action of the β-amyloid peptide on neurons, perhaps through protection against impaired glucose metabolism. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalRegulatory Peptides
Volume96
Issue number1-2
DOIs
StatePublished - 22 Dec 2000

Keywords

  • Activity-dependent neuroprotective protein
  • Activity-dependent neurotrophic factor
  • Cerebral cortical cultures
  • Vasoactive intestinal peptide

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