A novel monoclonal antibody, 1B3.1, binds to a new epitope of the VLA-1 molecule

Ilan Bank; Martin Hemler; Michael B. Brenner; Dina Cohen; Vered Levy; Jonathan Belko; Carol Crouse; Leonard Chess

doi:10.1016/0008-8749(89)90088-9

A novel monoclonal antibody, 1B3.1, binds to a new epitope of the VLA-1 molecule

Ilan Bank^*, Martin Hemler, Michael B. Brenner, Dina Cohen, Vered Levy, Jonathan Belko, Carol Crouse, Leonard Chess

^*Corresponding author for this work

Clinical Departments

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

A monoclonal antibody, 1B3.1, was raised against a cloned IL-2-dependent T cell line that expresses the TγδT cell receptor. MoAb 1B3.1 reacted with long-term cultured T cell lines of both Tγδ and Tαβ lineage, and with in vivo-stimulated T cells, derived from synovial fluid, but not with resting or short-term activated T cells, B cells, or macrophages. Immunoprecipitation of the 1B3.1 target antigens showed that 1B3.1 recognizes a 200/110 kDa molecule that is identical to the VLA-1 heterodimer precipitated by MoAb TS2/7. 1B3.1, however, binds to an epitope of VLA-1 that is distinct from the TS2/7 binding site. This new MoAb could be useful in further studies of the functions of VLA-1, and of the cells that express this molecule.

Original language	English
Pages (from-to)	416-423
Number of pages	8
Journal	Cellular Immunology
Volume	122
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(89)90088-9
State	Published - Sep 1989

Funding

Funders	Funder number
IsraelC ancerR esearchF oundationF
United States-IsraelB inational Science Foundation
National Institutes of Health
National Cancer Institute	R01CA042368
United States-Israel Binational Science Foundation

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10.1016/0008-8749(89)90088-9

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title = "A novel monoclonal antibody, 1B3.1, binds to a new epitope of the VLA-1 molecule",

abstract = "A monoclonal antibody, 1B3.1, was raised against a cloned IL-2-dependent T cell line that expresses the TγδT cell receptor. MoAb 1B3.1 reacted with long-term cultured T cell lines of both Tγδ and Tαβ lineage, and with in vivo-stimulated T cells, derived from synovial fluid, but not with resting or short-term activated T cells, B cells, or macrophages. Immunoprecipitation of the 1B3.1 target antigens showed that 1B3.1 recognizes a 200/110 kDa molecule that is identical to the VLA-1 heterodimer precipitated by MoAb TS2/7. 1B3.1, however, binds to an epitope of VLA-1 that is distinct from the TS2/7 binding site. This new MoAb could be useful in further studies of the functions of VLA-1, and of the cells that express this molecule.",

author = "Ilan Bank and Martin Hemler and Brenner, \{Michael B.\} and Dina Cohen and Vered Levy and Jonathan Belko and Carol Crouse and Leonard Chess",

note = "Funding Information: This work was supportedi n part by Grant 8600221 from the United States-IsraelB inational Science Foundation( BSF), Jerusalem,I sraelt o LB. and L.C., by an IsraelC ancerR esearchF oundationF ellowship Award to I.B., and by National Instituteso f Health Grant CA42368t o M.E.H.",

year = "1989",

month = sep,

doi = "10.1016/0008-8749(89)90088-9",

language = "אנגלית",

volume = "122",

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journal = "Cellular Immunology",

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T1 - A novel monoclonal antibody, 1B3.1, binds to a new epitope of the VLA-1 molecule

AU - Bank, Ilan

AU - Hemler, Martin

AU - Brenner, Michael B.

AU - Cohen, Dina

AU - Levy, Vered

AU - Belko, Jonathan

AU - Crouse, Carol

AU - Chess, Leonard

N1 - Funding Information: This work was supportedi n part by Grant 8600221 from the United States-IsraelB inational Science Foundation( BSF), Jerusalem,I sraelt o LB. and L.C., by an IsraelC ancerR esearchF oundationF ellowship Award to I.B., and by National Instituteso f Health Grant CA42368t o M.E.H.

PY - 1989/9

Y1 - 1989/9

N2 - A monoclonal antibody, 1B3.1, was raised against a cloned IL-2-dependent T cell line that expresses the TγδT cell receptor. MoAb 1B3.1 reacted with long-term cultured T cell lines of both Tγδ and Tαβ lineage, and with in vivo-stimulated T cells, derived from synovial fluid, but not with resting or short-term activated T cells, B cells, or macrophages. Immunoprecipitation of the 1B3.1 target antigens showed that 1B3.1 recognizes a 200/110 kDa molecule that is identical to the VLA-1 heterodimer precipitated by MoAb TS2/7. 1B3.1, however, binds to an epitope of VLA-1 that is distinct from the TS2/7 binding site. This new MoAb could be useful in further studies of the functions of VLA-1, and of the cells that express this molecule.

AB - A monoclonal antibody, 1B3.1, was raised against a cloned IL-2-dependent T cell line that expresses the TγδT cell receptor. MoAb 1B3.1 reacted with long-term cultured T cell lines of both Tγδ and Tαβ lineage, and with in vivo-stimulated T cells, derived from synovial fluid, but not with resting or short-term activated T cells, B cells, or macrophages. Immunoprecipitation of the 1B3.1 target antigens showed that 1B3.1 recognizes a 200/110 kDa molecule that is identical to the VLA-1 heterodimer precipitated by MoAb TS2/7. 1B3.1, however, binds to an epitope of VLA-1 that is distinct from the TS2/7 binding site. This new MoAb could be useful in further studies of the functions of VLA-1, and of the cells that express this molecule.

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A novel monoclonal antibody, 1B3.1, binds to a new epitope of the VLA-1 molecule

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