A novel microfluidic whole cell biosensor based on electrochemical detection for water toxicity analysis

H. Ben-Yoav, Y. Shacham-Diamand, A. Freeman, A. Biran, R. Pedahzur, S. Belkin, S. Buchinger, G. Reifferscheid

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Over the last few years, the physical dimensions of microchip devices have decreased, enhancing the interest in the integration of various devices and complex operations onto a compatible "lab on a chip" system with desirable characteristics and capabilities. This work presents a novel μ-fluidics whole cell biosensor for water toxicity analysis. The biosensor is based on bacterial cells genetically "tailored" to generate an electrochemical bio-signal in the presence of toxic materials. The μ-chip was electrochemically characterized, and demonstrated the potential toxicity analysis with a model toxicant. A novel concept of bacterial biosensors deposition by means of electrophoretic force was examined for the first time. Preliminary results demonstrated the ability to detect electrochemical signal generated by the deposited bacterial cells indicating the potential use for patterning of bacterial cells on solid-state surfaces for the use in bio-sensing.

Original languageEnglish
Title of host publicationECS Transactions - Chemical Sensors 8
Subtitle of host publicationChemical (Gas, Ion, Bio) Sensors and Analytical Systems
PublisherElectrochemical Society Inc.
Pages187-197
Number of pages11
Edition11
ISBN (Print)9781566776578
DOIs
StatePublished - 2009
EventChemical Sensors 8: Chemical (Gas, Ion, Bio) Sensors and Analytical Systems - 214th ECS Meeting - Honolulu, HI, United States
Duration: 12 Oct 200817 Oct 2008

Publication series

NameECS Transactions
Number11
Volume16
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Conference

ConferenceChemical Sensors 8: Chemical (Gas, Ion, Bio) Sensors and Analytical Systems - 214th ECS Meeting
Country/TerritoryUnited States
CityHonolulu, HI
Period12/10/0817/10/08

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