A non-compartmentalized glucose | O2 biofuel cell by bioengineered electrode surfaces

Eugenii Katz, Itamar Willner, Alexander B. Kotlyar

Research output: Contribution to journalArticlepeer-review

348 Scopus citations

Abstract

A novel glucose | O2 biofuel cell element was assembled by the engineering of layered bioelectrocatalytic electrodes. The anode consists of a surface reconstituted glucose oxidase monolayer, whereas the cathode is presented by the reconstituted cytochrome c/cytochrome oxidase couple. At the GOx monolayer-functionalized electrode, bioelectrocatalyzed oxidation of glucose to gluconic acid occurs, whereas at the Cyt. c | COx layered electrode, the reduction of O2 to water takes place. The alignment of the glucose oxidase monolayer on the electrode surface yields an extremely efficient electrical communication, and the electron transfer turnover rate between the redox-center and the conductive support leads to an oxygen insensitive enzyme electrode. This enables the operation of the biofuel cell without the compartmentalization of the anode and the cathode. The system paves the way to tailoring invasive biofuel cells for generating electrical power. The analysis of the interfacial electron transfer processes of the electrodes suggests that by appropriate genetic engineering of the proteins, and appropriate chemical architecturing of the redox-proteins on the electrodes, the extracted power of the biofuel cell element could be further improved.

Original languageEnglish
Pages (from-to)64-68
Number of pages5
JournalJournal of Electroanalytical Chemistry
Volume479
Issue number1
DOIs
StatePublished - 22 Dec 1999

Funding

FundersFunder number
Belfer Foundation

    Keywords

    • Biofuel cell
    • Cytochrome c
    • Electrochemistry
    • Enzyme catalysis
    • Monolayers

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