@article{14097b0ac75d494db342b7ce1ec19b19,
title = "A new visual screening assay for catalytic antibodies with retro-aldol retro-Michael activity",
abstract = "Fast and convenient methods are required for the detection of novel catalysts. We have developed a new assay to allow direct visualization of retro-aldol retro-Michael catalytic activity and have demonstrated it with catalytic antibody 38C2. The assay is based on a catalytic cleavage of a physiologically stable substrate to release 3,4-cyclohexeneoesculetin. The latter then reacts with iron(III) to generate a non-soluble complex that precipitates in the form of a black dye. This assay may be used for screening new catalysts for retro-aldol retro-Michael activity with improved efficiency for specific prodrug activation.",
keywords = "Aldol reaction, Assay, Catalytic antibodies, Prodrug activation",
author = "Marina Shamis and Barbas, \{Carlos F.\} and Doron Shabat",
note = "Funding Information: D.S. thanks the Israel Science Foundation, the Israel Ministry of Science {\textquoteleft}Tashtiot{\textquoteright} program, and the Israel Cancer Association for financial support. ",
year = "2007",
month = mar,
day = "1",
doi = "10.1016/j.bmcl.2006.12.057",
language = "אנגלית",
volume = "17",
pages = "1172--1175",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Elsevier Ltd.",
number = "5",
}