TY - JOUR
T1 - A new type of microglia gene targeting shows TAK1 to be pivotal in CNS autoimmune inflammation
AU - Goldmann, Tobias
AU - Wieghofer, Peter
AU - Müller, Philippe F.
AU - Wolf, Yochai
AU - Varol, Diana
AU - Yona, Simon
AU - Brendecke, Stefanie M.
AU - Kierdorf, Katrin
AU - Staszewski, Ori
AU - Datta, Moumita
AU - Luedde, Tom
AU - Heikenwalder, Mathias
AU - Jung, Steffen
AU - Prinz, Marco
N1 - Funding Information:
We thank M. Oberle, C. Fix and S. Gaupp for excellent technical assistance. Special thanks to M. Olschewski for help with the thorough statistical analysis of our data. M.P. was supported by the Bundesministerium für Bildung und Forschung–funded competence network of multiple sclerosis (KKNMS), the competence network of neurodegenerative disorders (KNDD), the Deutsche Forschungsgemeinschaft (SFB 992, FOR1336, PR 577/8-1), the Fritz-Thyssen Foundation, the Gemeinnützige Hertie Foundation (GHST) and Biogen Idec. P.F.M. was supported by an MD educational grant of the SFB620. M.H. was funded by the Helmholtz Foundation, the SFB-TR36, a European Research Council starting grant and the Helmholtz Alliance Preclinical Comprehensive Cancer Center. S.J. was supported by the Deutsche Forschungsgemeinschaft (FOR1336) and by the Israel Science Foundation.
PY - 2013/11
Y1 - 2013/11
N2 - Microglia are brain macrophages and, as such, key immune-competent cells that can respond to environmental changes. Understanding the mechanisms of microglia-specific responses during pathologies is hence vital for reducing disease burden. The definition of microglial functions has so far been hampered by the lack of genetic in vivo approaches that allow discrimination of microglia from closely related peripheral macrophage populations in the body. Here we introduce a mouse experimental system that specifically targets microglia to examine the role of a mitogen-associated protein kinase kinase kinase (MAP3K), transforming growth factor (TGF)-β-activated kinase 1 (TAK1), during autoimmune inflammation. Conditional depletion of TAK1 in microglia only, not in neuroectodermal cells, suppressed disease, significantly reduced CNS inflammation and diminished axonal and myelin damage by cell-autonomous inhibition of the NF-κB, JNK and ERK1/2 pathways. Thus, we found TAK1 to be pivotal in CNS autoimmunity, and we present a tool for future investigations of microglial function in the CNS.
AB - Microglia are brain macrophages and, as such, key immune-competent cells that can respond to environmental changes. Understanding the mechanisms of microglia-specific responses during pathologies is hence vital for reducing disease burden. The definition of microglial functions has so far been hampered by the lack of genetic in vivo approaches that allow discrimination of microglia from closely related peripheral macrophage populations in the body. Here we introduce a mouse experimental system that specifically targets microglia to examine the role of a mitogen-associated protein kinase kinase kinase (MAP3K), transforming growth factor (TGF)-β-activated kinase 1 (TAK1), during autoimmune inflammation. Conditional depletion of TAK1 in microglia only, not in neuroectodermal cells, suppressed disease, significantly reduced CNS inflammation and diminished axonal and myelin damage by cell-autonomous inhibition of the NF-κB, JNK and ERK1/2 pathways. Thus, we found TAK1 to be pivotal in CNS autoimmunity, and we present a tool for future investigations of microglial function in the CNS.
UR - http://www.scopus.com/inward/record.url?scp=84886953273&partnerID=8YFLogxK
U2 - 10.1038/nn.3531
DO - 10.1038/nn.3531
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C2 - 24077561
AN - SCOPUS:84886953273
SN - 1097-6256
VL - 16
SP - 1618
EP - 1626
JO - Nature Neuroscience
JF - Nature Neuroscience
IS - 11
ER -
