A new nuclear component of the Wnt signalling pathway

Barry Thompson; Fiona Townsley; Rina Rosin-Arbesfeld; Hannah Musisi; Mariann Bienz

doi:10.1038/ncb786

A new nuclear component of the Wnt signalling pathway

Barry Thompson
, Fiona Townsley
, Rina Rosin-Arbesfeld
, Hannah Musisi
, Mariann Bienz^*

^*Corresponding author for this work

Medical Research Council

Research output: Contribution to journal › Article › peer-review

262 Scopus citations

Abstract

The Wnt signalling pathway is pivotal in normal and malignant development. A key effector is Armadillo (Arm)/β-catenin, which functions with TCF to transcribe Wnt target-genes. Here, we report the discovery of pygopus (pygo), whose mutant phenotypes specifically mimic loss-of-Wingless (Wg) signalling. pygo is required for dTCF-mediated transcription, but not for Wg-induced stabilization of Arm. Pygo is a nuclear protein that is found in a complex with Arm in vivo. Humans possess two Pygo proteins, both of which are required for TCF-mediated transcription in colorectal cancer cells. The presence of a PHD domain implicates Pygo proteins in a chromatin-related function, and we propose that they mediate chromatin access to TCF or Arm/β-catenin.

Original language	English
Pages (from-to)	367-373
Number of pages	7
Journal	Nature Cell Biology
Volume	4
Issue number	5
DOIs	https://doi.org/10.1038/ncb786
State	Published - 2002
Externally published	Yes

Funding

Funders
EMBO

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1038/ncb786

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title = "A new nuclear component of the Wnt signalling pathway",

abstract = "The Wnt signalling pathway is pivotal in normal and malignant development. A key effector is Armadillo (Arm)/β-catenin, which functions with TCF to transcribe Wnt target-genes. Here, we report the discovery of pygopus (pygo), whose mutant phenotypes specifically mimic loss-of-Wingless (Wg) signalling. pygo is required for dTCF-mediated transcription, but not for Wg-induced stabilization of Arm. Pygo is a nuclear protein that is found in a complex with Arm in vivo. Humans possess two Pygo proteins, both of which are required for TCF-mediated transcription in colorectal cancer cells. The presence of a PHD domain implicates Pygo proteins in a chromatin-related function, and we propose that they mediate chromatin access to TCF or Arm/β-catenin.",

author = "Barry Thompson and Fiona Townsley and Rina Rosin-Arbesfeld and Hannah Musisi and Mariann Bienz",

note = "Funding Information: ACKNOWLEDGEMENTS We would like to thank A. Baonza, M. van Beest, C. Murawsky and M. Frasch for antibodies, B. Nichols and S. Munro for RNA oligomers, H. Clevers and S. Munro for plasmids, A. Baonza for technical advice on wing discs, and H. Pelham, M. Freeman and A. Travers for critical comments on the manuscript. Thanks also to R. Nusse for suggesting the name Pygopus, a legless and scaly lizard, reminiscent of the pygopus mutant phenotype. R.R.-A. is supported by an EMBO Long-term fellowship. Correspondence and requests for material should be addressed to M.B. Supplementary information is available on Nature Cell Biology{\textquoteright}s website (http://cellbio.nature.com).",

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AU - Townsley, Fiona

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N1 - Funding Information: ACKNOWLEDGEMENTS We would like to thank A. Baonza, M. van Beest, C. Murawsky and M. Frasch for antibodies, B. Nichols and S. Munro for RNA oligomers, H. Clevers and S. Munro for plasmids, A. Baonza for technical advice on wing discs, and H. Pelham, M. Freeman and A. Travers for critical comments on the manuscript. Thanks also to R. Nusse for suggesting the name Pygopus, a legless and scaly lizard, reminiscent of the pygopus mutant phenotype. R.R.-A. is supported by an EMBO Long-term fellowship. Correspondence and requests for material should be addressed to M.B. Supplementary information is available on Nature Cell Biology’s website (http://cellbio.nature.com).

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N2 - The Wnt signalling pathway is pivotal in normal and malignant development. A key effector is Armadillo (Arm)/β-catenin, which functions with TCF to transcribe Wnt target-genes. Here, we report the discovery of pygopus (pygo), whose mutant phenotypes specifically mimic loss-of-Wingless (Wg) signalling. pygo is required for dTCF-mediated transcription, but not for Wg-induced stabilization of Arm. Pygo is a nuclear protein that is found in a complex with Arm in vivo. Humans possess two Pygo proteins, both of which are required for TCF-mediated transcription in colorectal cancer cells. The presence of a PHD domain implicates Pygo proteins in a chromatin-related function, and we propose that they mediate chromatin access to TCF or Arm/β-catenin.

AB - The Wnt signalling pathway is pivotal in normal and malignant development. A key effector is Armadillo (Arm)/β-catenin, which functions with TCF to transcribe Wnt target-genes. Here, we report the discovery of pygopus (pygo), whose mutant phenotypes specifically mimic loss-of-Wingless (Wg) signalling. pygo is required for dTCF-mediated transcription, but not for Wg-induced stabilization of Arm. Pygo is a nuclear protein that is found in a complex with Arm in vivo. Humans possess two Pygo proteins, both of which are required for TCF-mediated transcription in colorectal cancer cells. The presence of a PHD domain implicates Pygo proteins in a chromatin-related function, and we propose that they mediate chromatin access to TCF or Arm/β-catenin.

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A new nuclear component of the Wnt signalling pathway

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UN SDGs

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