TY - JOUR
T1 - A new nuclear component of the Wnt signalling pathway
AU - Thompson, Barry
AU - Townsley, Fiona
AU - Rosin-Arbesfeld, Rina
AU - Musisi, Hannah
AU - Bienz, Mariann
N1 - Funding Information:
ACKNOWLEDGEMENTS We would like to thank A. Baonza, M. van Beest, C. Murawsky and M. Frasch for antibodies, B. Nichols and S. Munro for RNA oligomers, H. Clevers and S. Munro for plasmids, A. Baonza for technical advice on wing discs, and H. Pelham, M. Freeman and A. Travers for critical comments on the manuscript. Thanks also to R. Nusse for suggesting the name Pygopus, a legless and scaly lizard, reminiscent of the pygopus mutant phenotype. R.R.-A. is supported by an EMBO Long-term fellowship. Correspondence and requests for material should be addressed to M.B. Supplementary information is available on Nature Cell Biology’s website (http://cellbio.nature.com).
PY - 2002
Y1 - 2002
N2 - The Wnt signalling pathway is pivotal in normal and malignant development. A key effector is Armadillo (Arm)/β-catenin, which functions with TCF to transcribe Wnt target-genes. Here, we report the discovery of pygopus (pygo), whose mutant phenotypes specifically mimic loss-of-Wingless (Wg) signalling. pygo is required for dTCF-mediated transcription, but not for Wg-induced stabilization of Arm. Pygo is a nuclear protein that is found in a complex with Arm in vivo. Humans possess two Pygo proteins, both of which are required for TCF-mediated transcription in colorectal cancer cells. The presence of a PHD domain implicates Pygo proteins in a chromatin-related function, and we propose that they mediate chromatin access to TCF or Arm/β-catenin.
AB - The Wnt signalling pathway is pivotal in normal and malignant development. A key effector is Armadillo (Arm)/β-catenin, which functions with TCF to transcribe Wnt target-genes. Here, we report the discovery of pygopus (pygo), whose mutant phenotypes specifically mimic loss-of-Wingless (Wg) signalling. pygo is required for dTCF-mediated transcription, but not for Wg-induced stabilization of Arm. Pygo is a nuclear protein that is found in a complex with Arm in vivo. Humans possess two Pygo proteins, both of which are required for TCF-mediated transcription in colorectal cancer cells. The presence of a PHD domain implicates Pygo proteins in a chromatin-related function, and we propose that they mediate chromatin access to TCF or Arm/β-catenin.
UR - http://www.scopus.com/inward/record.url?scp=0036097733&partnerID=8YFLogxK
U2 - 10.1038/ncb786
DO - 10.1038/ncb786
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C2 - 11988739
AN - SCOPUS:0036097733
SN - 1465-7392
VL - 4
SP - 367
EP - 373
JO - Nature Cell Biology
JF - Nature Cell Biology
IS - 5
ER -
