A new method for the rapid measurement of cholesterol crystallization in model biles using a spectrophotometric microplate reader

Giora J. Sömjen, Yehuda Ringel, Fred M. Konikoff, Ruth Rosenberg, Tuvia Gilat

Research output: Contribution to journalArticlepeer-review

Abstract

Measurements of the cholesterol crystal observation time, and particularly the crystal growth rate in model biles, are important in biliary pathophysiology. The aim of this study was to develop a semi-automated method permitting multiple, simultaneous, and precise measurements of the crystal growth rate in model biles. Incubated model biles were mixed with a high concentration of NaTDC to solubilize non-crystalline turbidity and spectrophotometric measurements were performed. In parallel, samples were observed by light microscopy. The absorbance correlated linearly with the crystal mass and permitted quantitation of the crystal growth rate. Polarized light microscopy was more sensitive than spectrophotometry for determining the initial crystal observation time, while spectrophotometry was more precise and quantitative for measuring the crystal growth rate.

Original languageEnglish
Pages (from-to)1048-1052
Number of pages5
JournalJournal of Lipid Research
Volume38
Issue number5
StatePublished - May 1997

Keywords

  • Bile salts
  • Nucleation
  • Phospholipids

Fingerprint

Dive into the research topics of 'A new method for the rapid measurement of cholesterol crystallization in model biles using a spectrophotometric microplate reader'. Together they form a unique fingerprint.

Cite this