TY - JOUR
T1 - A new method for determination of serum cholestanol by high-performance liquid chromatography with ultraviolet detection
AU - Halperin, Gideon
AU - Elisaf, Moses
AU - Leitersdorf, Eran
AU - Harats, Dror
PY - 2000/6/9
Y1 - 2000/6/9
N2 - We developed a method for the determination of serum 5α-cholestan-3β-ol (cholestanol). The sterols were derivatized to the 4'-bromobenzenesulfonyl esters and heated in isopropanol. The cholesterol-4'-bromobenzenesulfonate was solvolyzed to cholesteryl isopropyl ether, but the derivatized cholestanol did not change and could be measured in a high-performance liquid chromatographic system equipped with a UV detector at 235 nm. On the other hand, the resulting cholesteryl isopropyl ether, having different absorbance and chromatographic mobility was not detected. This method was used for measuring cholestanol levels in patients with cerebrotendinous xanthomatosis (CTX), liver cirrhosis and serum from healthy control subjects. Reproducibility, linearity and recovery tests were done on 0.3 ml of serum samples containing >2 μg/ml cholestanol, using stigmastanol as an internal standard (I.S.). Determining cholestenol by this method can be used for diagnosis and follow-up of patients with CTX and various liver diseases. Copyright (C) 2000 Elsevier Science B.V.
AB - We developed a method for the determination of serum 5α-cholestan-3β-ol (cholestanol). The sterols were derivatized to the 4'-bromobenzenesulfonyl esters and heated in isopropanol. The cholesterol-4'-bromobenzenesulfonate was solvolyzed to cholesteryl isopropyl ether, but the derivatized cholestanol did not change and could be measured in a high-performance liquid chromatographic system equipped with a UV detector at 235 nm. On the other hand, the resulting cholesteryl isopropyl ether, having different absorbance and chromatographic mobility was not detected. This method was used for measuring cholestanol levels in patients with cerebrotendinous xanthomatosis (CTX), liver cirrhosis and serum from healthy control subjects. Reproducibility, linearity and recovery tests were done on 0.3 ml of serum samples containing >2 μg/ml cholestanol, using stigmastanol as an internal standard (I.S.). Determining cholestenol by this method can be used for diagnosis and follow-up of patients with CTX and various liver diseases. Copyright (C) 2000 Elsevier Science B.V.
KW - Cholestanol
UR - http://www.scopus.com/inward/record.url?scp=0034625682&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(00)00184-5
DO - 10.1016/S0378-4347(00)00184-5
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C2 - 10901139
AN - SCOPUS:0034625682
SN - 1387-2273
VL - 742
SP - 345
EP - 352
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 2
ER -