A new Ki-67 / E-cadherin cocktail reduces inter-observer variation of the calculated proliferative index

Golan Bubis, Ohad Hilly*, Roy Bubis, Marisa Halpern, Ariel Schwartz, Rumelia Koren, Lea Rath-Wolfson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The proliferative index in breast carcinoma is usually calculated by the percentage of the Ki-67 positive cells out of the total number of malignant cells. In order to reduce the inter-observer variability of the calculated proliferative index a cocktail of antibodies against E-Cadherin and Ki-67 (Ki/Cad Cocktail) is presented. The cocktail was applied on 59 cases of infiltrating duct carcinoma of breast and compared to the consecutive slides stained for Ki-67 alone. The Ki/Cad cocktail has the advantage that by adding the anti E-Cadherin antibody, all the malignant epithelial cells are highlighted and can be differentiated from other proliferating cells. Statistical analysis proved that the cocktail increases the inter-observer agreement from 89 % to 97 % as compared to the Ki-67 alone and also reduces the overlap between the cancer grades.

Original languageEnglish
Pages (from-to)875-879
Number of pages5
JournalPathology and Oncology Research
Volume19
Issue number4
DOIs
StatePublished - Oct 2013

Keywords

  • Cocktail immunostain
  • E-Cadherin
  • Infiltrating duct carcinoma of breast
  • Ki-67
  • Proliferative index

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