Abstract
The proliferative index in breast carcinoma is usually calculated by the percentage of the Ki-67 positive cells out of the total number of malignant cells. In order to reduce the inter-observer variability of the calculated proliferative index a cocktail of antibodies against E-Cadherin and Ki-67 (Ki/Cad Cocktail) is presented. The cocktail was applied on 59 cases of infiltrating duct carcinoma of breast and compared to the consecutive slides stained for Ki-67 alone. The Ki/Cad cocktail has the advantage that by adding the anti E-Cadherin antibody, all the malignant epithelial cells are highlighted and can be differentiated from other proliferating cells. Statistical analysis proved that the cocktail increases the inter-observer agreement from 89 % to 97 % as compared to the Ki-67 alone and also reduces the overlap between the cancer grades.
Original language | English |
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Pages (from-to) | 875-879 |
Number of pages | 5 |
Journal | Pathology and Oncology Research |
Volume | 19 |
Issue number | 4 |
DOIs | |
State | Published - Oct 2013 |
Keywords
- Cocktail immunostain
- E-Cadherin
- Infiltrating duct carcinoma of breast
- Ki-67
- Proliferative index