TY - JOUR
T1 - A new assay of deoxyribonucleases using as a substrate radioactively labeled DNA bound either directly or through anti-DNA antibodies to plastic depression plates
AU - Slor, Hanoch
N1 - Funding Information:
This research was supported in part by the Israel Cancer Association. We are very grateful to Dr. E.C. Friedberg of Stanford University, California, USA, for his gift of T*t U.V.-endonuclease, and to Dr. I.R. Lehman, also of Stanford, for donating DNA Polymerase I.
PY - 1975/5
Y1 - 1975/5
N2 - A simple and accurate method for the detection of Deoxyribonucleases is described. The DNA substrate (3H-labeled) is bound to DNA-binding sites in the "wells" of plastic depression plates either directly of via anti-DNA antibodies. Following incubation with the appropriate enzyme, the radioactivity released from the wells or left bound to the wells is determined. The method is suitable for enzymes which attack native DNA, single stranded DNA and modified DNA (e.g., u.v.-irradiated) as substrates.
AB - A simple and accurate method for the detection of Deoxyribonucleases is described. The DNA substrate (3H-labeled) is bound to DNA-binding sites in the "wells" of plastic depression plates either directly of via anti-DNA antibodies. Following incubation with the appropriate enzyme, the radioactivity released from the wells or left bound to the wells is determined. The method is suitable for enzymes which attack native DNA, single stranded DNA and modified DNA (e.g., u.v.-irradiated) as substrates.
UR - http://www.scopus.com/inward/record.url?scp=0016592076&partnerID=8YFLogxK
U2 - 10.1093/nar/2.5.745
DO - 10.1093/nar/2.5.745
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C2 - 1170555
AN - SCOPUS:0016592076
SN - 0305-1048
VL - 2
SP - 745
EP - 756
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 5
ER -