A monoclonal antibody to a cross-reactive idiotype on cationic human anti-DNA antibodies expressing λ light chains: A new reagent to identify a potentially differential pathogenic subset

A. Livneh, A. Halpern, D. Perkins, A. Lazo, B. Diamond

Research output: Contribution to journalArticlepeer-review

Abstract

Anti-double-stranded DNA antibodies are commonly found in the serum of patients with systemic lupus erythematosus (SLE). They are a heterogeneous group of antibodies thought to differ in pathogenicity. The degree of heterogeneity and the structural correlates of pathogenicity, however, remain poorly defined. To address these questions we have been generating anti-idiotypic antibodies to the anti-DNA antibodies found in the serum of SLE patients. In this paper we report the generation and characterization of a new murine monoclonal anti-idiotype, 8.12, that recognizes a subset of anti-DNA antibodies that is present in serum of approximately 50% of patients with SLE. The 8.12 anti-idiotype recognizes uniquely cationic anti-DNA antibodies, all of which express λ light chains. In murine models of SLE, it has been suggested that cationic anti-DNA antibodies are preferentially deposited in the kidney. It may be, therefore, that 8.12 recognizes a subset of anti-DNA antibodies of particular pathogenic significance.

Original languageEnglish
Pages (from-to)123-127
Number of pages5
JournalJournal of Immunology
Volume138
Issue number1
StatePublished - 1987
Externally publishedYes

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