A modified rapid filtration assay of glycogen synthase

Yoram Oron*, Joseph Larner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The filter paper assay of glycogen synthase according to Thomas et al.(J. A. Thomas, K. K. Schlender, and J. Larner, 1968, Anal. Biochem. 25, 486-489) has been modified to obtain results in a significantly shorter period of time with increased sensitivity and no loss in accuracy. The modified method is based on filtration on glass-fiber filter disks using a multiple filtration apparatus. The assay was examined on glycogen synthase activity present in muscle extracts and was found to be superior to the original assay procedure as regards reproducibility and time required for processing samples. The new method has been used in our laboratory for over 6 months.

Original languageEnglish
Pages (from-to)409-410
Number of pages2
JournalAnalytical Biochemistry
Volume94
Issue number2
DOIs
StatePublished - 15 Apr 1979
Externally publishedYes

Funding

FundersFunder number
U.S. Public Health Service

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