TY - JOUR
T1 - A microtest plate assay of functional excitatory amino acid receptors
AU - Eshhar, Nomi
AU - Lusky, Moshe
AU - David, Peer
AU - Teichberg, Vivian I.
N1 - Funding Information:
This work was supportedb y a grant from Makor Chemicals+V .I.T. holds the Louis and Florence Katz-Cohen professoriacl hair in neuropharmacology.
PY - 1989/6
Y1 - 1989/6
N2 - A microtest plate assay of functional excitatory amino acid receptors present on cultured chick embryo retinal cells has been developed. It is based on measurements of excitatory amino acid-mediated increase in 22Na+ influx into retinal cells adhering to each of the 96 wells of microtest plates. Dose-dependent responses to l-glutamate, kainate and N-methyl-d-aspartate but not to quisqualate can be measured. These responses are selectively inhibited by antagonists of excitatory amino acids. The assay is reliable, fast to perform and parsimonious in terms of the volume and thus of amount of the drug applied. It allows a single investigator to perform, in one day, measurements of the effects of known or putative glutamatergic ligands in more than 100 different conditions.
AB - A microtest plate assay of functional excitatory amino acid receptors present on cultured chick embryo retinal cells has been developed. It is based on measurements of excitatory amino acid-mediated increase in 22Na+ influx into retinal cells adhering to each of the 96 wells of microtest plates. Dose-dependent responses to l-glutamate, kainate and N-methyl-d-aspartate but not to quisqualate can be measured. These responses are selectively inhibited by antagonists of excitatory amino acids. The assay is reliable, fast to perform and parsimonious in terms of the volume and thus of amount of the drug applied. It allows a single investigator to perform, in one day, measurements of the effects of known or putative glutamatergic ligands in more than 100 different conditions.
KW - Cultured chick retinal cell
KW - Excitatory amino acid
KW - Microtest plate assay
UR - http://www.scopus.com/inward/record.url?scp=0024581149&partnerID=8YFLogxK
U2 - 10.1016/0165-0270(89)90041-1
DO - 10.1016/0165-0270(89)90041-1
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AN - SCOPUS:0024581149
SN - 0165-0270
VL - 28
SP - 229
EP - 233
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 3
ER -