A microtest plate assay of functional excitatory amino acid receptors

Nomi Eshhar, Moshe Lusky, Peer David, Vivian I. Teichberg

Research output: Contribution to journalArticlepeer-review

Abstract

A microtest plate assay of functional excitatory amino acid receptors present on cultured chick embryo retinal cells has been developed. It is based on measurements of excitatory amino acid-mediated increase in 22Na+ influx into retinal cells adhering to each of the 96 wells of microtest plates. Dose-dependent responses to l-glutamate, kainate and N-methyl-d-aspartate but not to quisqualate can be measured. These responses are selectively inhibited by antagonists of excitatory amino acids. The assay is reliable, fast to perform and parsimonious in terms of the volume and thus of amount of the drug applied. It allows a single investigator to perform, in one day, measurements of the effects of known or putative glutamatergic ligands in more than 100 different conditions.

Original languageEnglish
Pages (from-to)229-233
Number of pages5
JournalJournal of Neuroscience Methods
Volume28
Issue number3
DOIs
StatePublished - Jun 1989
Externally publishedYes

Keywords

  • Cultured chick retinal cell
  • Excitatory amino acid
  • Microtest plate assay

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