A method for obtaining high recovery of purified subcellular fractions of rat liver homogenate

Meir Lahav; Nili Schoenfeld; Orit Epstein; Abraham Atsmon

doi:10.1016/0003-2697(82)90563-2

A method for obtaining high recovery of purified subcellular fractions of rat liver homogenate

Meir Lahav^*, Nili Schoenfeld, Orit Epstein, Abraham Atsmon

^*Corresponding author for this work

Clinical Departments

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Abstract

A method for obtaining highly purified subcellular fractions of rat liver is described. The recovery of each fraction approaches 100%. The method is based on differential centrifugation and the use of appropriate concentrations of Triton X-100 and MgCl₂ at certain specific steps in the fractionation procedure.

Original language	English
Pages (from-to)	114-122
Number of pages	9
Journal	Analytical Biochemistry
Volume	121
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(82)90563-2
State	Published - 15 Mar 1982

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title = "A method for obtaining high recovery of purified subcellular fractions of rat liver homogenate",

abstract = "A method for obtaining highly purified subcellular fractions of rat liver is described. The recovery of each fraction approaches 100%. The method is based on differential centrifugation and the use of appropriate concentrations of Triton X-100 and MgCl2 at certain specific steps in the fractionation procedure.",

author = "Meir Lahav and Nili Schoenfeld and Orit Epstein and Abraham Atsmon",

year = "1982",

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doi = "10.1016/0003-2697(82)90563-2",

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AU - Lahav, Meir

AU - Schoenfeld, Nili

AU - Epstein, Orit

AU - Atsmon, Abraham

PY - 1982/3/15

Y1 - 1982/3/15

N2 - A method for obtaining highly purified subcellular fractions of rat liver is described. The recovery of each fraction approaches 100%. The method is based on differential centrifugation and the use of appropriate concentrations of Triton X-100 and MgCl2 at certain specific steps in the fractionation procedure.

AB - A method for obtaining highly purified subcellular fractions of rat liver is described. The recovery of each fraction approaches 100%. The method is based on differential centrifugation and the use of appropriate concentrations of Triton X-100 and MgCl2 at certain specific steps in the fractionation procedure.

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JO - Analytical Biochemistry

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A method for obtaining high recovery of purified subcellular fractions of rat liver homogenate

Abstract

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