A Method for Improvement of Sperm Quality of an Ejaculated Human Specimen Using Bovine Cervical Mucus as a Biological Filter

ARIE BERGMAN; GIDEON YEDWAB; MENACHEM P. DAVID; ZVI T. HOMONNAI; GEDALIA F. PAZ

doi:10.1002/j.1939-4640.1982.tb00669.x

A Method for Improvement of Sperm Quality of an Ejaculated Human Specimen Using Bovine Cervical Mucus as a Biological Filter

ARIE BERGMAN, GIDEON YEDWAB, MENACHEM P. DAVID, ZVI T. HOMONNAI, GEDALIA F. PAZ^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

A method is described for improving the quality of semen from subfertile men. Semen samples were filtered through bovine cervical mucus (collected at estrus) into spermatozoa‐free seminal plasma (from the same ejaculate following centrifugation, or from azoospermic donor ejaculates). Evaluations were performed after 2 to 4 hours of filtration at 34 C. The filtered spermatozoa found in the seminal plasma reservoir were characterized by a significant increase in the percentage of morphologically normal spermatozoa, higher sperm motility, and a higher percentage of living sperm. Sperm density was in the range deemed adequate for fertility. After filtration into human cervical mucus, sperm penetration tests were performed with good results, which could be improved by the addition of caffeine to the semen. This method was found to be reliable and to enable the use of the filtered spermatozoa for insemination (AIH). 1982 American Society of Andrology

Original language	English
Pages (from-to)	199-204
Number of pages	6
Journal	Journal of Andrology
Volume	3
Issue number	3
DOIs	https://doi.org/10.1002/j.1939-4640.1982.tb00669.x
State	Published - 1982
Externally published	Yes

Keywords

bovine cervical mucus
penetration test
teratozoospermia

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/j.1939-4640.1982.tb00669.x

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title = "A Method for Improvement of Sperm Quality of an Ejaculated Human Specimen Using Bovine Cervical Mucus as a Biological Filter",

abstract = "A method is described for improving the quality of semen from subfertile men. Semen samples were filtered through bovine cervical mucus (collected at estrus) into spermatozoa‐free seminal plasma (from the same ejaculate following centrifugation, or from azoospermic donor ejaculates). Evaluations were performed after 2 to 4 hours of filtration at 34 C. The filtered spermatozoa found in the seminal plasma reservoir were characterized by a significant increase in the percentage of morphologically normal spermatozoa, higher sperm motility, and a higher percentage of living sperm. Sperm density was in the range deemed adequate for fertility. After filtration into human cervical mucus, sperm penetration tests were performed with good results, which could be improved by the addition of caffeine to the semen. This method was found to be reliable and to enable the use of the filtered spermatozoa for insemination (AIH). 1982 American Society of Andrology",

keywords = "bovine cervical mucus, penetration test, teratozoospermia",

author = "ARIE BERGMAN and GIDEON YEDWAB and DAVID, {MENACHEM P.} and HOMONNAI, {ZVI T.} and PAZ, {GEDALIA F.}",

year = "1982",

doi = "10.1002/j.1939-4640.1982.tb00669.x",

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AU - YEDWAB, GIDEON

AU - DAVID, MENACHEM P.

AU - HOMONNAI, ZVI T.

AU - PAZ, GEDALIA F.

PY - 1982

Y1 - 1982

N2 - A method is described for improving the quality of semen from subfertile men. Semen samples were filtered through bovine cervical mucus (collected at estrus) into spermatozoa‐free seminal plasma (from the same ejaculate following centrifugation, or from azoospermic donor ejaculates). Evaluations were performed after 2 to 4 hours of filtration at 34 C. The filtered spermatozoa found in the seminal plasma reservoir were characterized by a significant increase in the percentage of morphologically normal spermatozoa, higher sperm motility, and a higher percentage of living sperm. Sperm density was in the range deemed adequate for fertility. After filtration into human cervical mucus, sperm penetration tests were performed with good results, which could be improved by the addition of caffeine to the semen. This method was found to be reliable and to enable the use of the filtered spermatozoa for insemination (AIH). 1982 American Society of Andrology

AB - A method is described for improving the quality of semen from subfertile men. Semen samples were filtered through bovine cervical mucus (collected at estrus) into spermatozoa‐free seminal plasma (from the same ejaculate following centrifugation, or from azoospermic donor ejaculates). Evaluations were performed after 2 to 4 hours of filtration at 34 C. The filtered spermatozoa found in the seminal plasma reservoir were characterized by a significant increase in the percentage of morphologically normal spermatozoa, higher sperm motility, and a higher percentage of living sperm. Sperm density was in the range deemed adequate for fertility. After filtration into human cervical mucus, sperm penetration tests were performed with good results, which could be improved by the addition of caffeine to the semen. This method was found to be reliable and to enable the use of the filtered spermatozoa for insemination (AIH). 1982 American Society of Andrology

KW - bovine cervical mucus

KW - penetration test

KW - teratozoospermia

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A Method for Improvement of Sperm Quality of an Ejaculated Human Specimen Using Bovine Cervical Mucus as a Biological Filter

Abstract

Keywords

UN SDGs

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