A human model of xenogeneic graft-versus-host disease in SCID mice engrafted with human peripheral blood lymphocytes

Jasbir S. Sandhu; Reginald Gorczynski; Baruch Shpitz; Steven Gallinger; Hai Phu Nguyen; Nobumichi Hozumi

doi:10.1097/00007890-199507000-00011

A human model of xenogeneic graft-versus-host disease in SCID mice engrafted with human peripheral blood lymphocytes

Jasbir S. Sandhu, Reginald Gorczynski, Baruch Shpitz, Steven Gallinger, Hai Phu Nguyen, Nobumichi Hozumi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Despite previous attempts there is currently no suitable animal model available for xenogeneic graft versus host disease (XGVHD) mediated via human immunocompetent cells. Recently, we have developed an efficient protocol to engraft SCID mice with human peripheral blood lymphocytes (Hu-PBLs). The engraft-ment efficiency is extremely high, such that 100% of Hu-PBL-SCID mice die of XGVHD within 4 weeks after engraftment with Hu-PBLs (3-5 × l0⁷ cells). A series of experiments was performed to investigate the mechanisms involved in the severe XGVHD. The results suggest that XGVHD was induced by human CD4₊ T cells, antixenogeneic (antimouse) antibodies, and lympho-kines. The SCID mouse model will be extremely valuable for the evaluation and development of immunosuppressive agents and transplantation protocols for human XGVHD.

Original language	English
Pages (from-to)	179-184
Number of pages	6
Journal	Transplantation
Volume	60
Issue number	2
DOIs	https://doi.org/10.1097/00007890-199507000-00011
State	Published - 27 Jul 1995
Externally published	Yes

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10.1097/00007890-199507000-00011

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title = "A human model of xenogeneic graft-versus-host disease in SCID mice engrafted with human peripheral blood lymphocytes",

abstract = "Despite previous attempts there is currently no suitable animal model available for xenogeneic graft versus host disease (XGVHD) mediated via human immunocompetent cells. Recently, we have developed an efficient protocol to engraft SCID mice with human peripheral blood lymphocytes (Hu-PBLs). The engraft-ment efficiency is extremely high, such that 100% of Hu-PBL-SCID mice die of XGVHD within 4 weeks after engraftment with Hu-PBLs (3-5 × l07 cells). A series of experiments was performed to investigate the mechanisms involved in the severe XGVHD. The results suggest that XGVHD was induced by human CD4+ T cells, antixenogeneic (antimouse) antibodies, and lympho-kines. The SCID mouse model will be extremely valuable for the evaluation and development of immunosuppressive agents and transplantation protocols for human XGVHD.",

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AU - Sandhu, Jasbir S.

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AU - Shpitz, Baruch

AU - Gallinger, Steven

AU - Nguyen, Hai Phu

AU - Hozumi, Nobumichi

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AB - Despite previous attempts there is currently no suitable animal model available for xenogeneic graft versus host disease (XGVHD) mediated via human immunocompetent cells. Recently, we have developed an efficient protocol to engraft SCID mice with human peripheral blood lymphocytes (Hu-PBLs). The engraft-ment efficiency is extremely high, such that 100% of Hu-PBL-SCID mice die of XGVHD within 4 weeks after engraftment with Hu-PBLs (3-5 × l07 cells). A series of experiments was performed to investigate the mechanisms involved in the severe XGVHD. The results suggest that XGVHD was induced by human CD4+ T cells, antixenogeneic (antimouse) antibodies, and lympho-kines. The SCID mouse model will be extremely valuable for the evaluation and development of immunosuppressive agents and transplantation protocols for human XGVHD.

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A human model of xenogeneic graft-versus-host disease in SCID mice engrafted with human peripheral blood lymphocytes

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