A Highly Selective and Sensitive Chemiluminescent Probe for Real-Time Monitoring of Hydrogen Peroxide in Cells and Animals

Sen Ye, Nir Hananya, Ori Green, Hansen Chen, Angela Qian Zhao, Jiangang Shen, Doron Shabat, Dan Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Selective and sensitive molecular probes for hydrogen peroxide (H2O2), which plays diverse roles in oxidative stress and redox signaling, are urgently needed to investigate the physiological and pathological effects of H2O2. A lack of reliable tools for in vivo imaging has hampered the development of H2O2 mediated therapeutics. By combining a specific tandem Payne/Dakin reaction with a chemiluminescent scaffold, H2O2-CL-510 was developed as a highly selective and sensitive probe for detection of H2O2 both in vitro and in vivo. A rapid 430-fold enhancement of chemiluminescence was triggered directly by H2O2 without any laser excitation. Arsenic trioxide induced oxidative damage in leukemia was successfully detected. In particular, cerebral ischemia-reperfusion injury-induced H2O2 fluxes were visualized in rat brains using H2O2-CL-510, providing a new chemical tool for real-time monitoring of H2O2 dynamics in living animals.

Original languageEnglish
Pages (from-to)14326-14330
Number of pages5
JournalAngewandte Chemie - International Edition
Volume59
Issue number34
DOIs
StatePublished - 17 Aug 2020

Keywords

  • bioimaging
  • chemiluminescence
  • fluorescent probes
  • hydrogen peroxide
  • imaging agents

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