TY - JOUR
T1 - A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length
AU - Askree, Syed H.
AU - Yehuda, Tal
AU - Smolikov, Sarit
AU - Gurevich, Raya
AU - Hawk, Joshua
AU - Coker, Carrie
AU - Krauskopf, Anat
AU - Kupiec, Martin
AU - McEachern, Michael J.
PY - 2004/6/8
Y1 - 2004/6/8
N2 - Telomeres are nucleoprotein structures present at the ends of eukaryotic chromosomes that play a central role in guarding the integrity of the genome by protecting chromosome ends from degradation and fusion. Length regulation is central to telomere function. To broaden our knowledge about the mechanisms that control telomere length, we have carried out a systematic examination of ≈4,800 haploid deletion mutants of Saccharomyces cerevisiae for telomere-length alterations. By using this screen, we have identified > 150 candidate genes not previously known to affect telomere length. In two-thirds of the identified mutants, short telomeres were observed; whereas in one-third, telomeres were lengthened. The genes identified are very diverse in their functions, but certain categories, including DNA and RNA metabolism, chromatin modification, and vacuolar traffic, are overrepresented. Our results greatly enlarge the number of known genes that affect telomere metabolism and will provide insights into how telomere function is linked to many other cellular processes.
AB - Telomeres are nucleoprotein structures present at the ends of eukaryotic chromosomes that play a central role in guarding the integrity of the genome by protecting chromosome ends from degradation and fusion. Length regulation is central to telomere function. To broaden our knowledge about the mechanisms that control telomere length, we have carried out a systematic examination of ≈4,800 haploid deletion mutants of Saccharomyces cerevisiae for telomere-length alterations. By using this screen, we have identified > 150 candidate genes not previously known to affect telomere length. In two-thirds of the identified mutants, short telomeres were observed; whereas in one-third, telomeres were lengthened. The genes identified are very diverse in their functions, but certain categories, including DNA and RNA metabolism, chromatin modification, and vacuolar traffic, are overrepresented. Our results greatly enlarge the number of known genes that affect telomere metabolism and will provide insights into how telomere function is linked to many other cellular processes.
UR - http://www.scopus.com/inward/record.url?scp=2942532256&partnerID=8YFLogxK
U2 - 10.1073/pnas.0401263101
DO - 10.1073/pnas.0401263101
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C2 - 15161972
AN - SCOPUS:2942532256
SN - 0027-8424
VL - 101
SP - 8658
EP - 8663
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 23
ER -